| 几种肿瘤免疫抑制因子作用的研究 |
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| 引用本文： | 郝天玲,张桂梅,冯作化,张慧,姜志尧. 几种肿瘤免疫抑制因子作用的研究[J]. 华中科技大学学报(医学版), 1995, 0(2) |
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| 作者姓名： | 郝天玲 张桂梅 冯作化 张慧 姜志尧 |
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| 作者单位： | 武汉同济医科大学实验医学研究中心医学分子生物学研究室，同济医科大学基础医学院卫生部呼吸系疾病重点实验室 |
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| 摘 要： | 通过体外细胞培养，采用ＭＴＴ法和ＮＯ测定法，观察了淋巴瘤（Ｒａｊｉ）、实体瘤（Ｓ_（１８０））、艾氏腹水瘤（ＥＡＣ）、肝癌（ＨＣ）４种肿瘤细胞产生的肿瘤免疫抑制因子（ＴＤＳＦ）对小鼠腹腔巨噬细胞产生ＮＯ和Ｔ淋巴细胞激活的影响。结果发现Ｒａｊｉ－ＴＤＳＦ和Ｓ_（１８０）－ＴＤＳＦ对巨噬细胞产生ＮＯ均有明显的促进作用（Ｐ＜０．０１，Ｐ＜０．０５）．而ＥＡＣ－ＴＤＳＦ和ＨＣ－ＴＤＳＦ则否（Ｐ＞０．０５），它们对Ｔ淋巴细胞激活的抑制作用Ｓ_（１８０）－ＴＤＳＦ和ＥＡＣ－ＴＤＳＦ可被大蒜素（ＤＡＴＳ）所拮抗，与无ＤＡＴＳ组比较抑制率明显下降（Ｐ＜０．０１）；而ＤＡＴＳ却不能拮抗Ｒａｊｉ－ＴＤＳＦ和ＨＣ－ＴＤＳＦ的抑制作用（Ｐ＞０．０５）。结果表明，不同肿瘤细胞产生的ＴＤＳＦ的作用及其机制可能是多样的。
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| 关 键 词： | 肿瘤；免疫抑制因子；一氧化氮；大蒜素；巨噬细胞；T－淋巴细胞 |
Comparison of Several Tumor-derived Immunosuppressive Factors |
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| Hao Tianling,Zhang Guimei,Feng Zuohua et al. Comparison of Several Tumor-derived Immunosuppressive Factors[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1995, 0(2) |
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| Authors: | Hao Tianling Zhang Guimei Feng Zuohua et al |
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| Abstract: | The effects of tumor-derived immunosuppressive factors produced by four tumor cell lines on T cell activation and production of NO by macrophages were investigated.The TDSFs produced by Raji cells and S180 cells can significantly augment the production of NO by macrophages(P<0.01,P<0.05),but the TDSFs produced by EAC cells and HC cells had no influence on the production of NO by macrophages(P>0.05).Diallyl trisulfide(DATS)can antagonize the inhibitory effects of S180-TDSF and EAC-TDSF on T cell activation as compared with the DATS-free groups(P<0.01)but had no antagonistic effects on the inhibitory action of Raji-TDSF and HCDTDSF compared with the DATS-free group(P>0.05).The results suggest that the TDSFs from different tumor cells inhibit the activation of T lymphocytes by different mechanisms. |
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| Keywords: | tumor immunosuppressive factor nitrogen oxide diallyl trisulfide macrophage T lymphocyte |
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