多重耐药鲍曼不动杆菌携带β-内酰胺类药物耐药基因研究

目的：调查临床分离的10株多重耐药鲍曼不动杆菌（MDR-Ab）对常用的氨基糖苷类、β-内酰胺类、氯霉素、喹诺酮类、四环素、磺胺类和利福平等抗菌药物的耐药情况及其携带β-内酰胺类药物耐药基因的存在状况进行调查。方法10株试验菌均分离自2012年1月至2013年12月该院住院患者痰液标本，排除某一患者重复分离菌株。采用K-B纸片扩散法测定21种抗菌药物的敏感性，并用聚合酶链反应（PCR）检测β-内酰胺酶类、金属β-内酰胺酶类、碳青酶烯类及AmpC酶类等耐药基因。结果该MDR-Ab对21种抗菌药物均为耐药，共检出TEM、OXA-2、ADC、CTX-M-1、CTX-M-2、IMP、OXA-24等耐药基因。结论多重耐药鲍曼不动杆菌可能对目前临床常用的β-内酰胺类药物存在着广泛耐药。

Survey on multi-drug resistant acinetobacter baumannii with beta lactam drugs drug resistant gene

Objective Survey on drug resistance situation of clinically separated 10 multi-drug resistant acinetobacter baumannii（MDR-Ab） for commonly-used beta lactam class,aminoglycosides,chloromycetin,quinolones,sulfonamides and rifampicin and sulfa,etc and existence condition of resistant gene of carried beta lactam drugs. Methods The 10 test bacteria of sputum specimen separated from the inpatients from Jan. 2012 to Dec. 2013 excluding re-separation from a patient was adopted K-B dif-fusion method to detect sensibility of 21 antimicrobial agents,and beta lactam drugs,metal beta la-ctam enzymes,carbapenms and AmpC enzymes. Results The MDR-Ab was resistant to 21 antibacterial drugs,detecting TEM,OXA-2,ADC,CTX-M-1,CTX-M-2、IMP、OXA-24 and so on. Conclusion Multi-drug resistant acinetobacter baumannii（MDR-Ab） may be extensively resistant to currently-used beta lactam drugs.