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目的探讨糖皮质激素受体α(GRα)和糖皮质激素受体β(GRβ)在原发性血小板减少性紫癜(ITP)糖皮质激素抵抗中的分子生物学机制。方法选择2003-01—2005-12在深圳市儿童医院血液内科住院的ITP患儿30例,将患儿分为2组:激素敏感组(18例)和激素抵抗组(12例),另选10例正常儿童作对照。应用RT-PCR法检测激素敏感组、激素抵抗组ITP患儿及10例正常儿童外周血单个核细胞GRα和GRβmRNA的表达水平,以探讨GRα和GRβ与ITP糖皮质激素抵抗的关系。结果(1)对照组、激素敏感组和激素抵抗组3组之间GRαmRNA的表达分别为(0.75±0.17)、(0.70±0.15)、(0.73±0.16)]差异无显著性,P>0.05;(2)对照组和激素敏感组的GRβmRNA分别为(0.13±0.03)、(0.15±0.04)]差异亦无显著性,P>0.05,但激素抵抗组的GRβmRNA水平为(0.39±0.12)]显著高于对照组和激素敏感组,P<0.01,差异具有统计学意义。结论ITP患儿糖皮质激素抵抗可能与GRβmRNA的表达亢进有关。

A study on the relationship between α and β-isoform of glucocorticoid receptors and glucocorticoid in resistant idiopathic thrombocytopenia purpura

?? AbstractObjectiveTo identify the relationship between the expression of ?? and ?a?isoforms of glucocorticoid receptors(GR)and response to glucocorticoid in the patients with idiopathic thrombocytopenia purpura (ITP). MethodsSemi??quantitative RT??PCR was used to detect the expression of GR??,?? in PBMC among 30 patients with ITP and 10 normal controls. Results(1)The expression of GR??mRNA in PBMC showed among the groups.(2)The expression of GR??mRNA in the resistant group of ITP was significantly higher than that in both the sensitive group and control group (P<0.01). ConclusionThe expression of GR??mRNA may play a great role in resisting glucocorticoid resistance in the patients with ITP.