儿童单纯性肥胖与纤维蛋白原Bβ-148C/T、Bβ448G/A基因多态性关系的研究

目的 研究纤维蛋白原Bβ-148C/T、Bβ448G/A 基因多态性与儿童单纯性肥胖的相关性,为防治儿童单纯性肥胖提供理论依据.方法 抽取空腹静脉血采用聚合酶链反应限制性酶切方法对纤维蛋白原Bβ-148C/T、Bβ448G/A位点的基因型进行测定.结果 发现Bβ-148C/T基因型的分布在单纯性肥胖组和正常体重组差异有统计学意义(CC 51/67,CT 47/37,TT 8/2,基因型分布P=0.03);儿童单纯性肥胖T等位基因频率明显高于健康对照组(C 149/171,T 63/41,等位基因频率P=0.02),而B13448G/A基因型及等位基因频率的分布在单纯性肥胖组和正常体重组差异无统计学意义(GG 61/69,AG41/32,AA4/5,G 163/70,A49/42,均为P0.05).结论 纤维蛋白原Bβ-148 C/T基因多态性与儿童单纯性肥胖有相关性.而Bβ448G/A基因多态性与儿童单纯性肥胖不相关.

Relationship between simple obesity and fibrinogen Bβ-148C/T and Bβ448G/A gene polymorphism in children

Objective To study the correlation between simple obesity and flbrinogen Bβ-148C/T and Bβ448G/A gene polymorphism in children and to provide theoretical basis for children's imple obesity prevention.Methods 5ml fasting blood sample was taken from each patient.PCR and restriction enzyme digestion were used to detect the fibrinogen Bβ-148C/T and Bβ448G/A gene genotype.Results The genotype distribution of Bβ-148C/T was significantly different between simple obesity and normal controls(CC51/67, CT47/37 ,TT8/2, P =0.03 );The allele frequency in the children with simple obesity was higher than that of the healthy controls{ C 149/171 ,T63/41 ,P = 0.02).However, there was no significance between two groups in genotype distribution of Bβ 448G/A and allele frequency (GG61/69, AG41/32, AA4/5, G163/70, A49/42, P > 0.05 ). Conclusion There is significant cor-relation between simple obesity and fibrinogen Bβ-148 gene polymorphism in children but not between simple obesityand fibrinogen Bβ 448G/A gene polymorphism.