B淋巴细胞微粒数目在慢性阻塞性肺疾病患者支气管肺泡灌洗液中的变化

目的 初步探讨B 淋巴细胞微粒（ BLMPs） 数目在慢性阻塞性肺疾病（ 简称慢阻肺） 患者支气管肺泡灌洗液（ BALF） 中的变化情况, 并分析其数目变化与疾病分期可能存在的关系。方法 选取2012 年3 月至2013 年3 月在西南医院、新桥医院、重庆医科大学第一附属医院呼吸科就诊的慢阻肺急性加重期患者33 例, 慢阻肺稳定期患者12 例以及西南医院健康体检中心或者纤维支气管镜中心的对照者31 例。分别采集BALF, 并将所采集的样本进行离心处理后, 用CD20 抗人抗体进行标记, 使用流式细胞技术检测每100 μL灌洗液中BLMPs 的数目。结果 BLMPs 数目在三组之间有显著差异（ P 〈0. 05） , 其中慢阻肺急性加重组BLMPs 数目低于对照组和慢阻肺稳定期组（ P 〈0. 05） ; 而慢阻肺稳定期组与对照组比较差异无统计学意义（ P 〉0. 05） 。BLMPs 的数目与吸烟、性别、年龄、体表面积无关（ P 〉0. 05） 。结论 BLMPs 的数目在慢阻肺患者BALF 中降低, 尤其在急性加重期降低更明显, BLMPs 数目的减少可能与疾病分期有关, 吸烟、性别、年龄、体表面积对其数目的变化无影响。

Changes of B Lymphocyte-derived Microparticles in Bronchoalveolar Lavage Fluid of Patients with COPD

Objective To explore the changes of the B lymphocyte-derived microparticles （ BLMPs） in the bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease（ COPD） , and analyze the correlation between BLMPs changes and the stages of the disease.
Methods 33 COPD patients in acute exacerbation and 12 COPD patients in stable phase in Southwest Hospital, Xinqiao Hospital, and First Affiliated Hospital of Chongqing Medical University betweenMarch 2012 and March 2013 were enrolled in the study. 31 subjects who underwent physical examination and bronchoscopy were recruited as control. The lavage fluid specimens were collected through fiberoptic bronchoscopy, then marked with the corresponding antibodies after centrifugation. The numbers of microparticles were analyzed by flow cytometry. Results The number of the BLMPs was significant different among three groups （ P 〈 0. 05） .Compared with the control group and the stable COPD group, the number of BLMPs in the AECOPD group was significantly reduced （ P 〈 0. 05） . Compared with the control group, the number of the BLMPs in the stable COPD group was reduced but with no significant difference （ P 〉0. 05） . The numbers of BLMPs had no correlation with the smoking history, gender, age and body surface area.Conclusions The number of BLMPs is reduced in COPD, especially in the acute exacerbation stage, so the reductions of the BLMPs may be associated with the stages of the disease. Smoking, gender, age, body surface area have no effect on the number of BLMPs.