| 刚地弓形虫Peroxiredoxin多克隆抗体的制备及鉴定 |
| |
| 引用本文： | 苏丹华,沈超群,吴亮,刘原,付涛,周家豪,姜旭淦,陈盛霞,曹建平. 刚地弓形虫Peroxiredoxin多克隆抗体的制备及鉴定[J]. 中国人兽共患病杂志, 2015, 31(12): 1089-1092. DOI: 10.3969/j.issn.1002-2694.2015.12.001 |
| |
| 作者姓名： | 苏丹华 沈超群 吴亮 刘原 付涛 周家豪 姜旭淦 陈盛霞 曹建平 |
| |
| 作者单位： | 1.江苏大学医学院,镇江 212013; 2.中国疾病预防控制中心寄生虫病预防控制所,上海 200025 |
| |
| 基金项目： | 国家自然科学基金(No.81301453),卫生部寄生虫病原与媒介生物学重点实验室开放课题(No; WSBKTKT201302),中国博士后科学基金特别资助(No.2015T80518),中国博士后科学基金(No.2014M561598),江苏省博士后科研计划(No.1402171C)和江苏大学高级人才启动基金(No.13JDG023,No.13JDG127)联合资助 |
| |
| 摘 要： | 目的 原核表达刚地弓形虫过氧化物氧化还原酶(peroxiredoxin,Prx)并制备多克隆抗体。方法 PCR技术扩增弓形虫cDNA中的prx基因,克隆至pET-28a(+)载体,构建prx/pET-28a(+)重组表达载体,转化至大肠埃希菌(E.coli)Rosetta中诱导表达。亲和层析纯化重组Prx蛋白,并制备兔多克隆抗体,蛋白印迹技术对多克隆抗体进行鉴定。结果 成功从弓形虫cDNA中扩增出prx目的基因,构建了prx/pET-28a(+)重组质粒,获得抗Prx重组蛋白的多克隆抗体。蛋白印迹技术检测出弓形虫Prx的特异性条带。结论 重组弓形虫Prx制备的多克隆抗体能检测Prx在弓形虫速殖子表达。
|
| 关 键 词： | 刚地弓形虫 过氧化物氧化还原酶 原核表达 多克隆抗体 |
| 收稿时间： | 2015-04-01 |
Preparation and identification of polyclonal antibody of peroxiredoxin from Toxoplasma gondii |
| |
| SU Dan-hua,SHEN Chao-qun,WU Liang,LIU Yuan,FU Tao,ZHOU Jia-hao,JIANG Xu-gan,CHEN Sheng-xia,CAO Jian-ping. Preparation and identification of polyclonal antibody of peroxiredoxin from Toxoplasma gondii[J]. Chinese Journal of Zoonoses, 2015, 31(12): 1089-1092. DOI: 10.3969/j.issn.1002-2694.2015.12.001 |
| |
| Authors: | SU Dan-hua SHEN Chao-qun WU Liang LIU Yuan FU Tao ZHOU Jia-hao JIANG Xu-gan CHEN Sheng-xia CAO Jian-ping |
| |
| Affiliation: | 1.School of Medicine, Jiangsu University, Zhenjiang 212013, China; 2.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China |
| |
| Abstract: | To clone and express the peroxiredoxin(Prx) of Toxoplasma gondii RH strain and produce the polyclonal antibody, prx gene fragments amplified by PCR were cloned into the pET-28a(+) vector to construct the prokaryotic expression vector prx/pET-28a(+), which was efficiently expressed in E.coli Rosetta. Recombinant Prx proteins were purified by Ni-NTA affinity chromatography. The anti-Prx polyclonal antibody was produced in rabbit. Western blotting could detect Prx expression by anti-Prx polyclonal antibody. The recombinant Prx polyclonal antibody will facilitate the study of the biological functions of Prx. |
| |
| Keywords: | Toxoplasma gondii peroxiredoxin (Prx) prokaryotic expression polyclonal antibody |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国人兽共患病杂志》浏览原始摘要信息 |
|
点击此处可从《中国人兽共患病杂志》下载全文 |
|
