低分子肝素微乳及其纳米脂质体作大鼠口服抗凝效果的比较

目的　制备低分子肝素纳米脂质体及微乳制剂,并进行大鼠口服吸收后抗凝效果的比较。方法　用逆相蒸发法制备低分子肝素纳米脂质体;按三元相图摸索微乳区;以涡旋振荡制备低分子肝素微乳,马尔文激光粒度分析仪测量粒径,以大鼠用药前后血液凝固时间的变化研究其口服吸收的效果。结果　低分子肝素纳米脂质体平均粒径为84 . 1nm ,包封率为2 3 .6 %,微乳平均粒径5 2 . 8nm ;大鼠灌胃微乳及脂质体后,血液凝固时间显著延长。结论　纳米脂质体及微乳对低分子肝素大鼠口服吸收均有良好的促进作用,微乳效果好于纳米脂质体。

Comparation of Anticoagulation Effects of Low Molecular Weight Heparin(LMWH) Nano-liposomes and Microemulsions in Rat by Oral Administration

AIM Preparing nano-liposomes and microemusions of Low Molecular Weight Heparin and comparing their anticoagulation effects after oral administration.METHODS Making LMWH nano-liposomes by methods of reversing phase evaporation, searching the regions of microemulsions by using pseudo-ternary phase diagram and making microemulsions by votex mixer; Measuring particle sizes by Malvern Zetasizer 3000 HSA, Studying the absorption results by the differences of coagulation times of rats before and after oral administration.RESULTS The average particle sizes of nano-liposomes is 84.1 nm and the encapsulation percentage is 23.6%.The average particle sizes of microemulsions is 52.8 nm.The coagulation time is prolonged significantly.CONCLUSION Nano-liposomes and microemulsions could both enhance the intestine absorptions of LMWH very well, but microemulsions are better.