分子生物学方法鉴定鲍曼不动杆菌与仪器鉴定结果比较

目的 比较鲍曼不动杆菌的分子生物学鉴定结果和仪器鉴定结果。方法 采用法国梅里埃公司VITEK2COMPACT全自动微生物分析仪对细菌进行鉴定和抗菌药物敏感性分析,鉴定为鲍曼不动杆菌复合群的细菌,采用聚合酶链反应（PCR）检测OXA-51基因。结果 68株VITEK2 COMPACT鉴定为鲍曼不动杆菌复合群的菌株中,50株OXA-51基因阳性,确定为鲍曼不动杆菌;18株OXA-51基因阴性,为复合群其它细菌。鲍曼不动杆菌（OXA-51阳性）与复合群其它细菌（OXA-51阴性）比较,对临床常用的碳青酶烯类、氨基糖苷类、氟喹诺酮类、头孢菌素类等抗菌药物的敏感性降低（P〈0.05）。结论 鲍曼不动杆菌复合群其它细菌耐药率显著低于鲍曼不动杆菌,采用检测OXA-51基因方法对鲍曼不动杆菌复合群进行准确的菌种鉴定有利于正确指导临床抗菌药物的选择和感染控制的介入。

Evaluation of Molecular Biology and Instrument Identification of Acinetobacter Baumannii Strains

Objective To Evaluate the value of molecular biology and instrument identification of Acinetobacter bauman-nii strains. Methods Acinetobacter baumannii-A. calcoaceticus complex identification and drug resistance rate was detected using VITEK2 COMPACT of biomerieux. The drug resistance gene OXA-51 was detected by polymerase chain reaction assay. Results Of 68 isolates selected for analysis, 50（73. 53%） were OXA-51-like positive. These isolates were confirmed to be Acinetobacter baumannii. 18 of the 68 isolates were OXA-51-like negative. Comparing with OXA-51-like positive Acinetobacter baumannii, the resistance rates of OXA-51-like negative isolates to carbapenems, aminoglycosides, fluoroquinolones, and cephems were decreased （ P〈0. 05 ） . Conclusion Acinetobacter baumannii was more resistant to antibiotics than other Acinetobacter baumannii-A. calcoaceticus complex isolates. It is important for us to identify Acinetobacter baumannii exactly by detecting OXA-51-like gene in order to guide the clinical selection of antibiotics and nosocomial infection control.