二十二碳六烯酸增加人胃癌细胞SGC-7901对多柔比星的敏感性

目的:本研究旨在探讨二十二碳六烯酸[docosahexaenoic acid (DHA),22:6 ω-3]逆转由多柔比星( adriamycin,ADR)引起的胃癌SGC-7901细胞的耐药性.方法:体外常规培养人低分化胃腺癌细胞SGC-7901,分别以DHA、ADR单药和DHA+ADR联合作用于细胞24 h后,采用MTT法检测各组细胞的增殖情况；倒置光学显微镜下观察细胞的形态；FCM法检测细胞的凋亡率；激光共聚焦显微镜以及高效液相色谱法检测细胞中ADR的蓄积情况；RT-PCR及蛋白质印迹法检测细胞中多药耐药蛋白(multidrug resistanceprotein,MDR) P-糖蛋白p-170的表达情况.结果:DHA与ADR联合作用于胃癌SGC-7901细胞后,DHA能够增加ADR对细胞的生长抑制作用；细胞形态观察发现死细胞明显增多；细胞凋亡率显著增加；细胞中的ADR蓄积量约增加2.1倍；细胞中p-170 mRNA以及蛋白表达降低(P均＜0.05).结论:DHA能够增加胃癌细胞SGC-7901对ADR的敏感性,放大ADR的肿瘤杀伤作用.

Docosahexaenoic acid enhances the sensitivity of human gastric cancer SGC-7901 cells to adriamycin

Objective:To investigate the reversal effect of DHA (docosahexaenoic acid) on ADR (adriamycin)-induced multidrug resistance of human gastric cancer SGC-7901 cells. Methods:Human gastric cancer SGC-7901 cells were cultured in vitro and treated with either DHA or ADR alone or in combination for 24 h. The cell proliferation was detected by MTT method. The cell morphology was observed under an inverted microscope. The apoptotic rate was examined by FCM (flow cytometry). The cellular level of ADR was detected by laser scanning confocal microscopy and HPLC (high-performance liquid chromatography). The expression levels of MDR (multidrug resistance protein)-associated P-glycoprotein p-170 mRNA and protein were detected by RT-PCR and Western blotting, respectively. Results:The findings revealed that when DHA was given in combination with ADR, DHA significantly inhibited the growth of SGC-7901 cells. An increased number of dead cells and a higher apoptotic rate were observed. The intracellular accumulation of ADR was increased by 2.1-fold. The intracellular expressions of p-170 mRNA and protein were decreased (P < 0.05). Conclusion:DHA may enhance the sensitivity of gastric cancer SGC-7901 cells to ADR, so it can increase the antitumor effect of ADR.