B7-H1和Bcl-2在人结直肠癌中表达及其临床意义

目的:分析B7-H1及Bcl-2蛋白在人结直肠癌中的表达,并探讨其与结直肠癌临床病理特征之间的关系.方法:用免疫组织化学法检测57例手术切除的结直肠癌组织标本及癌旁正常组织标本中B7-H1及Bcl-2的表达,并结合临床病理特征进行相关性分析.结果:与癌旁正常组织相比,B7-H1及Bcl-2在结直肠癌组织中的阳性表达率明显高于癌旁组织,差异有统计学意义(P＜0.05).Bcl-2及B7-H1在结直肠癌组织中的表达均与分化程度呈负相关(P＜0.01),而且B7-H1在有淋巴结转移组(Dukes分期C+D)与无淋巴结转移组(Dukes分期A+B)相比,阳性表达率升高,差异有统计学意义(P＜0.05).另外,在高-中分化的结直肠癌组织中,B7-H1阴性组Bcl-2阳性表达率明显高于B7-H1阳性组,差异有统计学意义(P＜0.01).短期随访中,死亡的患者均B7-H1表达阳性.结论:B7-H1表达与结直肠癌的侵袭转移及生存期相关,且在高-中分化肿瘤组织中,提示与Bcl-2联合检测可能对结直肠癌的诊断、治疗及预后评价有一定的临床意义.

The expressions of B7-H1 and Bcl-2 in human colorectal cancer and their clinical signi cance

Objective: To investigate the expressions of B7-H1 and Bcl-2 proteins in human colorectal cancer, and to explore the association of the expressions of B7-H1 and Bcl-2 with clinicopathological features. Methods: The expressions of B7-H1 and Bcl-2 proteins were detected by immunohistochemistry in colorectal cancer tissues and their corresponding para-cancerous normal tissues from 57 cases of colorectal cancer. The association of the expressions of B7-H1 and Bcl-2 with the clinicopathological features was analyzed. Results: The positive expression rates of B7-H1 and Bcl-2 in colorectal cancer tissues were both higher than those in para-cancerous normal tissues (P <0.05). Both of Bcl-2 and B7-H1 expressions in colorectal cancer tissues were negatively correlated with the degree of differentiation of colorectal cancer (P <0.01). The positive expression rate of B7-H1 was significantly elevated in patients with lymph node metastasis (Dukes’ stage C-D) compared to the patients without lymph node metastasis (Dukes’ stage A-B) (P <0.05). For the patients with colorectal cancer classified as well or moderately differentiated, the expression rate of Bcl-2 in patients with negative expression of B7-H1 was significantly higher than that in patients with positive expression of B7-H1 (P <0.01). Short-term follow- up showed that the B7-H1 was expressed in colorectal cancer tissues from the patients died of colorectal cancer. Conclusion: The expression of B7-H1 was correlated with the invasion and metastasis of colorectal cancer as well as the survival. The detection of B7-H1 combined with Bcl-2 may have an important clinical significance in the diagnosis, treatment and prognostic prediction for patients with well/moderately differentiated colorectal cancer.