多发性硬化复发、缓解期血清MMP-9、TIMP-1含量的变化

目的探讨血清基质金属蛋白酶-9(MMP-9)及其组织抑制剂-1(TIMP-1)水平与缓解-复发型多发性硬化(RRMS)的关系并评价其临床意义。方法选择RRMS复发期患者28例、缓解期患者20例,并选择正常体检者30例为对照组。用酶联免疫吸附法(ELISA)检测血清MMP-9、TIMP-1水平,并进行统计学分析。结果 RRMS复发期患者血清MMP-9水平、MMP-9/TIMP-1比值较对照组显著升高(P<0.01),较缓解期患者也有明显升高(P<0.05),TIMP-1水平较对照组降低明显(P<0.05),但与缓解期患者比较差别无统计学意义(P=0.655)。RRMS复发期患者血清MMP-9水平、MMP-9/TIMP-1比值与EDSS评分无相关性。结论血清MMP-9含量、MMP-9/TIMP-1比值与RRMS的炎症活动密切相关,可作为监测RRMS活动性的血清学指标。

The Changes of Serum MMP-9 and TIMP-1 Concentrations on the Acute Exacerbation Stage and the Remission Stage of Multiple Sclerosis

Objective To investigate the changes of serum MMP-9 and TIMP-1 concentrations and their correlations with expanded disability status scale(EDSS) on the acute exacerbation stage and the remission stage of multiple sclerosis.To explore their values in the evaluation of RRMS activity.Methods 28 patients of RRMS in relapsing state(relapsing RRMS group),20 in remitting state(remitting RRMS group)and 30 healthy controls were enrolled.Their serum MMP-9 and TIMP-1 concentrations were detected by enzyme-linked-immunoassay(ELISA).Results The MMP-9 serum level and the ratio of MMP-9/TIMP-1 of the relapsing RRMS group were significantly higher than that of the healthy control group(P <0.01) and the remitting RRMS group(P<0.05).The TIMP-1 serum level of the relapsing RRMS group was significantly lower than that of the healthy control group(P<0.05) and had no significant difference with the remitting RRMS group(P=0.655).The MMP-9 serum level and the ratio of MMP-9/TIMP-1 of the relapsing RRMS group were not correlated with EDSS of MS.Conclusions The MMP-9 serum level and the ratio of MMP-9/TIMP-1 of the RRMS may be a biomarker reflecting the activity of disease.