不同冻存保护剂对脐血造血细胞生物学特性的影响

目的 探索有效低温保存脐血造血细胞的冻存保护剂。方法 对比４种不同的联合低温保护地脐血有核细胞（ＴＮＣ）的保护作用,并在冻存后１,２,３及４个月分别检测其复苏后脐血ＣＤＥ３４＾＋细胞及集落形成细胞（ＣＦＣ）数。结果 ４种不同联合的低温保护剂对脐血ＴＮＣ,ＣＤ３４＾＋细胞及ＣＦＣ的保护作用判别显,依次为右旋糖酐－４０（Ｄｅｘｔｒａｎ－４０）＋二甲基亚砜（ＤＭＳＯ）〉生理盐水＋ＤＭＳＯ〉ＤＭＳＯ＋自

Effects of different cryoprotectants on biological properties of hematopoietic cells derived from cord blood

Objective To search for effective cryoprotectants that can be used in cord blood bank to cryopreserve hematopoietic cells. Methods Four kinds of combined reagents were used to cryopreserve cord blood cells. The effects of these cryoprotectants on biological properties of hematopoietic cells were monitored and compared after the cells were refrigerated for 1 to 4 months. The detected biological properties included the number of total nucleated cells(TNC), CD 34 cells and CFCs. Results The cryoprotectants of Dextran 40 10%DMSO resulted in the highest recovery rates, TNC, CD 34 cells and CFCs were 77.8%, 78.5% and 87.2%, respectively. The combination of 10%DMSO 6% AB serum 5%HES led to the lowest recovery rates. Conclusion Dextran 40 10%DMSO is an ideal cryoprotectant combination for cryopreserving hematopoietic cells in the cord blood bank.