人脐血造血细胞扩增后表面标志的动态研究

目的 探讨脐血造血细胞体外扩增及其移植的最佳时机。方法 用粒细胞集落刺激因子、粒－巨噬细胞集落刺激因子、重组人白细胞介素３、重组白细胞介素６、干细胞因子和红细胞生成素长期培养脐血造血细胞，并对其表面标志及细胞增殖周期进行动态分析。结果 脐血中ＣＤ３＾＋细胞、ＣＤ４＾＋／ＣＤ４５ＲＯ＾＋和ＣＤ８＾＋／ＣＤ４５ＲＯ＾＋细胞培养３ｄ稍有增加，培养７ｄ达高峰，１４ｄ显下降，培养２１ｄ时处于更低水平。ＣＤ

Analysis of surface markers of expanded human umbilical cord blood cells in vitro

OBJECTIVE: To explore the optimal timing for in vitro expansion and transplantation of umbilical cord blood hematopoietic cells. METHODS: Hematopoietic cells from human umbilical cord blood were cultured with G-CSF, GM-CSF, rIL-3, rIL-6, SCF and Epo in a long term culture system, the cell cycle and cellular markers were analysed dynamically. RESULTS: CD(3)(+), CD(4)(+)/CD(45RO)(+) and CD(8)(+)/CD(45RO)(+) cells increased at day 3, and reached the highest level at day 7, decreased at day 14, and remained lower level at day 21. CD(4)(+) and CD(8)(+) cells showed no change at day 7, decreased at day 14, and decreased further at day 21. CD(4)(+)/CD(45RA)(+) and CD(8)(+)/CD(45RA)(+) cells decreased after day 3, and decreased further at day 14. HLA-DR(+) cells increased from day 3 to day 7. CD(34)(+) cells increased at day , reached the highest level at day 7, and decreased at day 14. CD(34)(+), CD(34)(+) CD(38)(-), CD(34)(+) CD(38)(+) cells increased 8.8, 7.4 and 8.7 fold at day 7, respectively, as compared with that precultured. The cells in the S phase increased at day 21. CONCLUSIONS: This culture system is able to expand hematopoietic cells of human umbilical cord blood and keeps them intact. The number of hematopoietic cells reached the highest level in the culture from day 7 to day 14, which is the optimal time for transplantation.