地氟烷对全脑缺血再灌注损伤大鼠超微结构的影响

背景地氟烷可减少大鼠不完全脑缺血模型神经功能的缺失,然而,形态学上地氟烷脑保护作用的证据尚不充足.目的研究地氟烷对全脑缺血再灌注损伤大鼠超微结构的影响,探讨地氟烷的脑保护作用.设计随机对照的实验研究.单位首都医科大学附属北京天坛医院.材料在首都医科大学附属北京神经外科研究所对9只Wistar大鼠进行研究.随机分为3组,缺血组(n=3),地氟烷组(n=3)和假手术对照组(n=3).干预制备大鼠全脑缺血再灌注模型.地氟烷组再灌注开始后立即吸入地氟烷1 h.缺血组和地氟烷组于缺血再灌注1 h、假手术组于手术后1 h取脑,利用电镜技术观察皮质超微结构的变化.主要观察指标皮质超微结构变化.结果与缺血组比较,地氟烷组神经元固缩小,神经元的细胞器和微管结构基本正常,但胶质细胞和微循环的破坏并无改善.结论地氟烷对神经元、细胞骨架可能具有保护作用,但对胶质细胞和微循环可能无保护作用.

Effect of desflurane on ultrastructure in rats with global cerebral ischemia-reperfusion injury

BACKGROUND:Desflurane can decrease the hyponeuria of incomplete cerebral ischemia-reperfusion of rat models. But there is not enough evidence to show morphologically its brain protective effect.OBJECTIVE: To observe the influence of desflurane on the ultrastructure of global cerebral ischemia-reperfusion in rats and study its effect on brain protection.DESIGN: Randomized and controlled study.SETTING: Beijing Tiantan Hospital of Capital University of Medical Sciences.MATERIALS: The experiment was carried out in the Beijing Institute of Neurosurgery of Capital Medical University. The nine Wistar rats were randomized into 3 groups: ischemia group( n = 3), desflurane group( n = 3),and sham-operation group( n=3).INTERVENTIONS: Rat models with global cerebral ischemia-reperfusion were prapared. The desflurane inhalation for one hour was given immediately to the desflurane group when the reperfusion started. The brain was obtained one hour after the ischemia-reperfusion in the ischemia and desflurane groups and after the operation in the sham-operation group. The changes of cortical ultrastructure were observed with electron microscope.MAIN OUTCOME MEASURES: The changes of cortical ultrastructure .RESULTS: The pyknosis of neurons in thedesflurane group was less, and the organelle and microtubulo-structure were basically normal, but the gliocytes and microcirculation were not improved, as compared with those of the ischemia group .CONCLUSION: Desflurane may have protective effects on neurons and cytoskeletons, but not on the gliocytes and microcirculations.