地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响

目的:研究地黄饮子水煎剂对去势大鼠骨强度和骨病理形态结构的影响,进一步探讨其作用机制。方法:采用去卵巢法制备大鼠骨质疏松症模型,将大鼠随机分为假手术组,模型组,地黄饮子水煎剂低、中、高剂量组,西药组(戊酸雌二醇片),每组10只,分别予相应药物灌胃4周,股动脉采血处死大鼠。观察大鼠的一般状况,称量体重,比色分析法检测血清钙(calcium,Ca),磷(phosphorus,P)含量,酶联免疫吸附法(ELISA)检测血清骨钙素(osteocalcin,BGP)含量和抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,tr ACP)含量,双能X射线吸收测量仪平台上测定右股骨部位骨密度(bone mineral density,BMD),用AG-IX生物力学万能实验机检测股骨最大载荷,定位-伸长位移及苏木素-伊红(HE)染色法观察股骨的病理形态学变化。结果:与假手术组比较,模型组大鼠BMD,Ca,P,BGP,最大载荷,定伸长-位移均显著降低(P0.05),而模型组大鼠体重和tr ACP的含量及地黄饮子水煎剂各干预组大鼠体重则显著增加(P0.05),同时模型组骨小梁结构不完整、丢失、断裂严重。与模型组相比,地黄饮子水煎剂各干预组大鼠BMD,BGP,Ca,最大载荷,定伸长-位移均显著升高(P0.05),其高、中剂量组大鼠P含量显著升高,而其高剂量组大鼠体重和tr ACP的含量则显著降低(P0.05),并且地黄饮子水煎剂高剂量组骨小梁排列规则,数目增多、增宽。结论:地黄饮子能够提高绝经后骨质疏松大鼠的骨密度,促进骨形成、抑制骨吸收,对绝经后骨代谢异常具有积极的防治作用。

Effect of Dihuang Yinzi Decoction on Bone Strength and Femur Pathological Morphology in Rats by Ovariectomy

Objective: To study the effects of Dihuang Yinzi decoction on bone strength and femur pathological morphology of ovariectomized rats, to further explore its mechanism. Method: Cutting off ovaris on both sides made rat model of osteoporosis, all animals were randomly separated into sham operation group, model control group, Dihuang Yinzi decoction high, medium, low dose group, western medicine control group(estradiol valerate tablets), with 10 rats in each group. The rats were gavaged with corresponding drugs for 4 weeks. And weight method was monitor the weight body of each rats. The general statu of the rats was evalued, the contents of Ca and P was used to check by colorimetric analysis, the contents of BGP and trACP were used to check by ELISA, bone mineral density(BMD) was used to obtain data by X ray dual energy absorptiometer, maximum load and fixed elongation displacement was used to obtain data by AG-IX biomechanical universal testing machine, the morphological changes of the femur was used to observe by HE staining. Result: Compared with those of sham operation group, the BMD,Ca,P,BGP,maximum load and fixed elongation displacement were all decreased obviously (P<0.05), but the weight body and trACP activity were both markedly increased (P<0.05), as well as, trabecular bone structure was incomplete,missing and broken seriously. Compared with those of model control group, the BMD,Ca,P,BGP,maximum load and fixed elongation displacement were all raised sharply in dihuangyinzi decoction treatment group, the P content was increased sharply in dihuangyinzi decoction high,middle dose group(P<0.05), but the weight body and trACP activity were both significantly slowed in Dihuang Yinzi decoction high dose group (P<0.05), meantime, bone trabecula was arranged regularly, bone trabecula was increased in number and widthin Dihuang Yinzi decoction high dose group. Conclusion: Dihuang Yinzi could improve bone density and inhibit bone formation, reduce bone absorption in rats with postmenopausal osteoporosis, and lead to a positive effect on the treatment of postmenopausal abnormal bone metabolism.