Hemozoin as a novel adjuvant for inactivated whole virion influenza vaccine |
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| Authors: | Ryuta Uraki Subash C. Das Masato Hatta Maki Kiso Kiyoko Iwatsuki-Horimoto Makoto Ozawa Cevayir Coban Ken J. Ishii Yoshihiro Kawaoka |
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| Affiliation: | 1. Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan;2. Influenza Research Institute, Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53711, USA;3. Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan;4. Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan;5. Laboratory of Malaria Immunology, Immunology Frontier Research Center (IFReC), Osaka University, Osaka, Japan;6. Laboratory of Adjuvant Innovation, National Institute of Biomedical Innovation, Osaka, Japan;g Laboratory of Vaccine Science, IFReC, Osaka University, Osaka, Japan;h ERATO Infection-Induced Host Responses Project (JST), Saitama 332-0012, Japan;i Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan |
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| Abstract: | Because vaccination is an effective means to protect humans from influenza viruses, extensive efforts have been made to develop not only new vaccines, but also for new adjuvants to enhance the efficacy of existing inactivated vaccines. Here, we examined the adjuvanticity of synthetic hemozoin, a synthetic version of the malarial by-product hemozoin, on the vaccine efficacy of inactivated whole influenza viruses in a mouse model. We found that mice immunized twice with hemozoin-adjuvanted inactivated A/California/04/2009 (H1N1pdm09) or A/Vietnam/1203/2004 (H5N1) virus elicited higher virus-specific antibody responses than did mice immunized with non-adjuvanted counterparts. Furthermore, mice immunized with hemozoin-adjuvanted inactivated viruses were better protected from lethal challenge with influenza viruses than were mice immunized with non-adjuvanted inactivated vaccines. Our results show that hemozoin improves the immunogenicity of inactivated influenza viruses, and is thus a promising adjuvant for inactivated whole virion influenza vaccines. |
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| Keywords: | Influenza virus Vaccine Hemozoin Adjuvant Antibody |
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