Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits |
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Authors: | Guanghui Li Xi Wang Jian Cao Zhaoyu Ju Dongyang Ma Yanpu Liu Junrui Zhang |
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Affiliation: | 1. Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi’an 710032, China;2. Department of Stomatology, PLA 252 Hospital, Baoding 071000, China;3. Department of Orthodontics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;4. Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou 730000, China |
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Abstract: | The reconstruction of large bony defects remains a clinical challenge, and angiogenesis and neovascularisation are being given more attention in bone tissue engineering. In this study we cocultured peripheral blood CD34+ cells (PB-CD34+ cells), an endothelial progenitor cell/haematopoietic stem cell-enriched population, with bone marrow-derived mesenchymal stem cells (MSC) to investigate their potential for bony regeneration. Cocultured cells showed better osteogenic differentiation than MSC alone in vitro. The cocultured cells and MSC sheets were also composited with hydroxyapatite and implanted in calvarial critical-size defects in rabbits. The rabbits were killed before microcomputed tomographic (MicroCT) and histological analysis. The results showed that cocultured cell composites had promoted bony regeneration more efficiently by 8 weeks after implantation. Our results indicate that the coculture of PB-CD34+ cells and MSC increases bony regeneration in calvarial critical-size defects in rabbits, and provide a new promising therapeutic strategy to aid skeletal healing. |
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Keywords: | Peripheral blood CD34+ cells Mesenchymal stem cells Co-culture Bone regeneration Rabbit calvarial critical-size defect Cell sheet transplantation |
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