18β-甘草次酸钠对小鼠腹腔巨噬细胞摄取氧化型低密度脂蛋白的影响及其作用机制研究

目的:观察18β-甘草次酸钠(β-SG)对体外培养的小鼠腹腔巨噬细胞摄取氧化型低密度脂蛋白(oxLDL)的影响,并探讨其可能的作用机制。方法:收集并培养小鼠腹腔巨噬细胞,分为对照组、oxLDL组及oxLDL+β-SG(1、10、100μmol/L)组,经oxLDL(除对照组外)作用后测定细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)含量,观察不同浓度β-SG对巨噬细胞摄取oxLDL的影响;同时,测定细胞内丙二醛(MDA)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性。结果:oxLDL能显著增加巨噬细胞内TC、CE的含量,而预先加入β-SG的oxLDL组能剂量依赖性地抑制细胞内TC、CE含量的升高(P<0.01或P<0.05),FC各组无显著性差异(P>0.05);与oxLDL组比较,oxLDL+β-SG各组还能减少细胞内MDA含量,增加SOD和GSH-PX活性(P<0.01或P<0.05)。结论:β-SG能显著抑制体外培养的小鼠腹腔巨噬细胞对oxLDL的摄取,此作用可能与其能提高巨噬细胞的抗氧化能力有关。

Effect of 18β - Sodium Glycyrrhetate on Mice Peritoneal Macrophages'''' Uptake of OX - LDL and Mechanism Study

OBJECTIVE:To study the effect of 18β-sodium glycyrrhetate(β-SG)on the uptake of oxidized low density lipoprotein(oxLDL)in peritoneal macrophages of mice cultured in vitro and to study its possible mechanism.METHODS:Peripheral macrophages of mice were cultured and collected,then divided into control group,oxLDL group and oxLDL plus β-SG(1,10 and 100μmol/L)group.The contents of total cholesterol(TC),free cholesterol(FC)in macrophages after being treated with oxLDL(with the exception of the control group)were determined;and the effects of β-SG at different concentrations on macrophages' intake of oxLDL were monitored,meanwhile the level of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in macrophages were determined.RESULTS:The contents of TC and CE in macrophages were increased significantly by oxLDL,however,which were inhibited dose-dependently in macrophages in oxLDL group pre-treated with β-SG(P<0.01 or P<0.05).There was no significant difference among FC groups(P>0.05).As compared with oxLDL group,oxLDL plus β-SG group decreased MDA content in macrophages and increased the activities of SOD and GSH-PX(P<0.01 or P<0.05).CONCLUSION:β-SG can significantly inhibit mice peripheral macrophages' uptake of oxLDL,this might be associated to its action in enhancing of peripheral macrophages' anti-oxidation.