NDRG1和E-cadherin蛋白在直肠腺癌中表达意义的研究

目的 探讨N-myc下游调节基因（N-myc downstream-regulated gene-1，NDRG1)的编码蛋白NDRG1和E-cadherin（钙粘素）蛋白在直肠腺癌中的表达及其临床病理意义。方法 应用免疫组化法检测80例直肠腺癌和12例直肠腺瘤组织中NDRG1和E-cadherin蛋白的表达情况。结果 ⑴直肠腺癌和直肠腺瘤组织中NDRG1阳性表达率分别为77.5％ 和50.0％ ，E-cadherin蛋白阳性表达率分别为53.8％ 和91.7％ ；⑵在TNMⅢ～Ⅳ期和淋巴结转移组，NDRG1蛋白阳性表达率为95.0％，明显高于TNMⅠ～Ⅱ期和无淋巴结转移组的60.0％；在浆膜和浆膜外浸润组，NDRG1蛋_白阳性表达率为82.8％，高于肌层以内浸润组的63.6％，统计学处理，差异有显著性(P＜0.05)；E-cadherin蛋白阳性表达率在高-中分化直肠腺癌组为58.8％，明显高于低分化组的25.0％；而在TNMⅢ～Ⅳ期和有淋巴结转移组，为32.5％，低于TNMⅠ～Ⅱ期和无淋巴结转移组75.0％，差异有统计学意义(P＜0.05)。结论 NDRG1可能作为潜在癌基因在直肠腺癌进展过程中起一定作用，检测NDRG1和E-cadherin蛋白的表达有助于判断直肠腺癌生物学行为及恶性程度。

Expression and significance of NDRG1 and E-cadherin proteins in rectal adenocarcinoma

【Abstract】 Objective To investigate the clinical patholgical significance of NDRG1 and E-cadherin proteins expression in rectal adenocarcinoma． Methods The expressions of NDRG1 and E-cadherin proteins were detected by immunohisto-chemical technique in 80 cases of rectal adenocarcinoma and 12 cases of rectal adenoma tissues. Results ① the positive rates of NDRG1 protein were 77.5％(62／80) and 50.0％(6/12) respectively in rectal adenocarcinoma and the positive rates of E-cadherin protein were 53.8％(43/80) and 91.7％(11/12) respectively in rectal adenoma; .②In the group of lymph node metastasis and stage of TNMⅢ-Ⅳ,the positive rate of NDRG1 was 95.0％(38/40cases) and significantly higher than that in the group of without lymph node metastasis and stage TNMⅠ-Ⅱ(60.0％, 24/40cases)(P＜0.05) ; In the group of invasion to serosa or ectoplast, the positive rate of NDRG1 was 82.8％(48/58cases) and significantly higher than that in the group of invasion within the muscular layer (63.6％，14/22cases)(P＜0.05). In the group of the well-moderately differentiated rectal Adenocarcinoma, the positive rate of E-cadherin was 58.8％(40/68cases) and significantly higher than that in the group of the Poorly differentiated rectal Adenocarcinoma(25.0％,3/12cases); while in the group of lymph node metastasis and stage TNMⅢ-Ⅳ, that was 32.5％(13/40cases) and significantly lower than that in the group of without lymph node metastasis and stage TNMⅠ-Ⅱ(75.0％,30/40cases)(P＜0.05). Conclusion It was suggested that NDRG1 might be contribute to the progression of rectal adenocarcinoma as a potential oncogene, and investigation of the expression of NDRG1 and E-cadherin proteins might be advantageous to the judgment of biological behaviors and malignant degree in rectal adenocarcinoma.