三氧化二砷对人肝癌细胞黏附和侵袭影响的实验研究

目的探讨三氧化二砷(亚砷酸，As2O3)注射液是否具有抗肝癌细胞黏附和侵袭的作用及其相关机制。方法选用人类肝癌细胞株SMMC-7721细胞及裸鼠人类肝癌转移模型为研究对象，通过细胞运动迁移实验、细胞黏附实验和免疫组化方法观察As2O3对SMMC-7721细胞的运动迁移、与纤维粘连蛋白(FN)、内皮细胞黏附以及裸鼠人肝癌转移模型肝移植瘤表达CD44和MMP-2基因蛋白的影响。结果As2O3注射液可显著抑制SMMC-772l细胞在FN上的迁移运动和SMMC-7721细胞与FN、内皮细胞的黏附，并能降低裸鼠肝移植瘤CD44、MMP-2的表达。结论As2O3具有抗肝癌细胞黏附和侵袭的作用，其机制可能与As2O3能降低肝癌细胞表达CD44和MMP-2有关。

Study on Effect of Arsenic Trioxide on Adhesion and Invasion of Human Hepatocarcinoma Cells in Vitro

OBJECTIVE: To explore that the arsenic trioxide injection (ATI) has the effect in antagonizing adhesion and invasion of human hepatocarcinoma cells (HCC), and its relevant mechanism. METHODS: Human hepatocellular carcinoma cell line SMMC-7721 and the high metastatic nude mice human HCC in situ transplantation model was taken as the objects of study, the effects of ATI on the SMMC-7721 cell movement and migration, its adhesion with fibronectin (FN) and endothelial cell (EC), as well as the CD44 and MMP-2 gene protein expression in transplanted tumor of the model mice were observed by means of cell movement and migration test, cell adhesion test and immunohistochemical method. RESULTS: ATI could significantly inhibit SMMC-7721 cell movement and migration on FN, adhesion with FN and EC, also could lower CD44 and MMP-2 in cancer cells. CONCLUSION: ATI has the effects of antagonizing hepatocarcinoma cell adhesion and invasion, the mechanism may be related with the action of ATI in lowering CD44 and MMP-2 expression in cancer cells.