阿托伐他汀对冠状动脉微栓塞后心肌炎症的影响

目的 探讨阿托伐他汀干预治疗对冠状动脉微栓塞（CME）后心肌炎症的影响.方法 48只清洁级雄性SD大鼠经左心室内注射自体微血栓,同时短暂夹闭主动脉,建立大鼠CME模型后,随机分为未治疗组及阿托伐他汀（Atrovastatin）干预组,于术后7 d处死.HE染色观察梗死心肌,免疫组织化学染色及Western blot检测肿瘤坏死因子α（TNF-α）、白介素6（IL-6）及细胞间黏附因子1（ICAM-1）等炎症因子在心肌中表达的变化.结果 阿托伐他汀可轻度减少梗死面积,显著抑制CME后心肌中白细胞浸润及TNF-α、IL-6、ICAM-1 蛋白表达（P均〈0.05）.结论 阿托伐他汀能显著抑制CME后心肌炎症反应.

Roles of atrovastatin in the myocardial inflammation following coronary microembolization

Objective To investigate the roles of atrovastatin in the myocardial inflammation following coronary microembolization. Methods CME model in 48 rats was created by injection homologous microthrombotic particle suspension into left ventricle when clamping the ascending aorta. The CME rats were randomized to untreated group and atrovastatin treated group （n=24 respectively）. The rats were sacrificed on day 7 post-operationally. The morphological characteristics were evaluated in sections with HE staining and the protein expressions of TNF-~, IL-6 and ICAM-1 were semi-quantitatively analyzed by immunohistochemistry and Western blot. Results Atrovastatin slightly reduced infarct size and significantly suppressed inflammatory cell infiltration and the protein expression of TNF-α, IL-6 and ICAM-1 （P〈0.05）. Conclusion Atrovastatin markedly suppressed the TNF-α, IL-6 protein expression and effectively ameliorated myocardial inflammation.