| 羊水细胞培养技术的临床应用 |
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| 引用本文： | 王辉林,蓝慧娟,麦光兴,李启运,熊礼宽.羊水细胞培养技术的临床应用[J].国际检验医学杂志,2013,34(18):2374-2375. |
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| 作者姓名： | 王辉林 蓝慧娟 麦光兴 李启运 熊礼宽 |
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| 作者单位： | 王辉林 (深圳市宝安区妇幼保健院中心实验室,广东深圳,518133)； 蓝慧娟 (深圳市宝安区妇幼保健院中心实验室,广东深圳,518133)； 麦光兴 (深圳市宝安区妇幼保健院中心实验室,广东深圳,518133)； 李启运 (深圳市宝安区妇幼保健院中心实验室,广东深圳,518133)； 熊礼宽 (深圳市宝安区妇幼保健院中心实验室,广东深圳,518133)； |
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| 基金项目： | 深圳市宝安区科技计划项目(项目编号：2010559) |
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| 摘 要： | 目的探索羊水细胞染色体培养改良方法,提高培养、收获成功率,满足临床诊断需要。方法对493例羊水标本进行细胞培养和染色体收获,并对实验细节进行优化,建立标准操作程序。结果羊水培养成功率99.8%（492/493）,发现21-三体6例、18-三体2例、其他异常2例。结论采取严格质量控制;保留换液后的上清,解决因收获失败无法诊断的问题;采取肝素抗凝等,消除母血细胞干扰,可使羊水细胞培养成功。
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| 关 键 词： | 细胞 培养的 核型分析 产前诊断 染色体 羊水 |
Clinical application of amniotic fluid cell culture technology |
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| Wang Huilin,Lan Huijuan,Mai Guangxing,Li Qiyun,Xiong Likuan.Clinical application of amniotic fluid cell culture technology[J].International Journal of Laboratory Medicine,2013,34(18):2374-2375. |
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| Authors: | Wang Huilin Lan Huijuan Mai Guangxing Li Qiyun Xiong Likuan |
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| Institution: | (Central Laboratory , Bao＇an District Maternal and Child health Hospital, Shenzhen, Guangdong 51813 3, China ) |
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| Abstract: | Objective To improve the success rate of cell culture and harvesting,by exploring the modified culture methods of amniotic fluid cell,and satisfy the demand for clinical diagnosis.Methods Conducting cell culture and chromosome harvest on 493 cases of amniotic fluid specimens,optimizing the experimental details and establishing the standard operation process.Results The success rate of amniotic fluid cell culture was 99.8%（492/493）,moreover,6cases of 21-trisome,2cases of 18-trisome and 2cases of other abnormalities were shown in the tests.Conclusion To ensure the success of amniotic fluid cell culture,it is important to take strict quality control,retain the supernatant after substituting medium for solving the undiagnosed problem caused by harvest failure,and eliminate maternal cell interference by heparin anticoagulant. |
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| Keywords: | cells cultured karyotyping prenatal diagnosis chromosomes amniotic fluid |
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