A cyclin protein modulates mitosis in the budding yeast Saccharomyces cerevisiae |
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Authors: | Lela M Veinot-Drebot Gerald C Johnston Richard A Singer |
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Institution: | (1) Department of Biochemistry, Dalhousie University, B3H 4H7 Halifax, Nova Scotia, Canada;(2) Department of Microbiology, Dalhousie University, B3H 4H7 Halifax, Nova Scotia, Canada;(3) Department of Medicine, Dalhousie University, B3H 4H7 Halifax, Nova Scotia, Canada;(4) Present address: Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9 Toronto, Ontario, Canada |
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Abstract: | Summary For the budding yeast Saccharomyces cerevisiae the mitotic cell cycle is coordinated with cell mass at the regulatory step start. The threshold amount of cell mass (reflected as a critical size) necessary for start is proportional to nutrient quality. This relationship leads to a transient accumulation of cells at start, termed nutrient modulation, upon enrichment of nutrient conditions. Nutrient enrichment abruptly increases the critical size needed for start, causing the smaller cells, produced in the previous cell cycle, to be delayed at start while growing larger. Here we show that, in S. cerevisiae, a second cell-cycle step, at mitosis, also exhibits nutrient modulation, and is, therefore, another point of cell-cycle regulation. At both mitosis and start, nutrient modulation was found through mutation to be regulated by the activity of the cyclin-related WHI1 (CLN3) gene product. |
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Keywords: | Nutrient modulation Critical size Cell cycle Startgif" alt="ldquo" align="MIDDLE" BORDER="0">Start" target="_blank">gif" alt="rdquo" align="MIDDLE" BORDER="0"> |
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