Ras-p21, p53在阴茎癌腹股沟淋巴结中的表达及临床意义

目的探讨阴茎癌腹股沟淋巴结ras-p21, p53基因蛋白的表达及临床意义。方法 取收治的阴茎癌44例, 随机切取腹股沟淋巴结44枚, 病理证实为转移癌的18枚, 炎性26枚;另选取同期腹股沟感染淋巴结20枚, 正常淋巴结20枚。石蜡切片, S-P染色。检测ras-p21, p53基因蛋白的表达。结果 ras-p21, p53的表达在阴茎癌腹股沟淋巴结组与对照组(正常淋巴结组、非瘤炎性淋巴结组)相比显著增高(P＜0.05)。腹股沟淋巴结癌转移阳性组较阴性组ras-p21, p53的表达显著增高(P＜0.05)。结论 在淋巴结病理检查的同时行p21蛋白和p53蛋白检测可提高阴茎癌转移诊断率。ras-p21蛋白和p53基因蛋白检测可以作为肿瘤是否转移和预后效果判定的客观指标。可做为阴茎癌是否行腹股沟淋巴结清除手术的判断指标。

The expression and clinical significance of ras-p21,p53 gene protein in inguinal lymphnodes with penis carcinoma

Objective To investigate the expression and clinical significance of ras-p21, p53 gene protein in inguinal lymph nodes with penis carcinoma.Methods The clinical data of 44 cases of perfect penis carcinoma from 1990 to 2002, selected 44 of inguinal lymph nodes of them at random, pathological examination showed that 18 cases had metastatic carcinoma, 26 were inflammatory affection.Separately, selected 20 of nonspecific inflammatory inguinal lymph nodes synchronously, 20 of normal lymph nodes, paraffin-embedded, sectioned, SP stained, examined the expression of ras-p21, p53 gene protein.Results The expression of ras-p21, p53 gene protein in the group of inguinal lymph nodes with penis carcinoma is significantly higher than those in normal lymph nodes and nonspecific inflammatory lymph nodes, it is positive correlation with tumor clinical stage(P＜0.05).Cancerometastasis of inguinal lymph nodes is significant different compared with the control group(P＜0.05).Conclusions It can improve the diagnosis rate to detect ras-p21, p53 gene protein at the same time of lymph nodes biopsy.The detection of ras-p21 and p53 gene protein can be the Objective target to criterion metastasis and prognosis.And so, it can be the decision index whether the elimination of inguinal lymph nodes is necessary.