| 重组RANK蛋白抑制破骨细胞活性的实验研究 |
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| 引用本文： | 张里程,张立海,黄鹏,张巍,唐佩福. 重组RANK蛋白抑制破骨细胞活性的实验研究[J]. 军医进修学院学报, 2010, 31(6): 584-586 |
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| 作者姓名： | 张里程 张立海 黄鹏 张巍 唐佩福 |
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| 作者单位： | 解放军总医院,骨科,北京,100853 |
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| 基金项目： | 国家自然科学基金项目(30672135);;北京市自然科学基金项目(7072072)~~ |
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| 摘 要： | 目的研究重组RANK蛋白在体外实验中对破骨细胞的抑制作用。方法成骨细胞与RAW264.7细胞以4∶1比例混合培养6d,以抗酒石酸酸性磷酸酶（TRAP）染色鉴定后,加入3种浓度（10-4g/L、10-5g/L、10-6g/L）重组RANK蛋白,并设空白对照。3d后观察破骨细胞数目和形态,计数骨磨片吸收陷窝。结果混合培养6d后,体系中可见成熟破骨细胞出现。加重组RANK蛋白3d后,与对照组相比,各药物组TRAP阳性细胞个数、骨磨片吸收陷窝计数均明显减少。结论体外实验中重组RANK蛋白可以显著抑制破骨细胞活性及吸收功能。
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| 关 键 词： | 破骨细胞 骨吸收类疾病 重组RANK蛋白 |
Recombinant RANK protein inhibits absorption activity of osteoclasts |
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| ZHANG Li-cheng,ZHANG Li-hai,HUANG Peng,ZHANG Wei,TANG Pei-fu. Recombinant RANK protein inhibits absorption activity of osteoclasts[J]. Academic Journal of Pla Postgraduate Medical School, 2010, 31(6): 584-586 |
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| Authors: | ZHANG Li-cheng ZHANG Li-hai HUANG Peng ZHANG Wei TANG Pei-fu |
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| Affiliation: | ZHANG Li-cheng,ZHANG Li-hai,HUANG Peng,ZHANG Wei,TANG Pei-fu Department of Orthopedics,Chinese PLA General Hospital,Beijing 100853,China |
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| Abstract: | Objective To study the inhibitory effect of recombinant RANK protein on absorption activity of osteoclasts in vitro.Methods Mouse osteoblasts and RAW264.7 cells were cocultured in 6-well plates at the ratio of 4 to 1 for 6 days.The cells were stained with tartrate-resistant acid phosphatase(TRAP).Three days after recombinant RANK protein was added at the concentrations of 10-4g/L,10-5g/L and 10-6g/L and the control was set,the quantity and morphology of osteoclasts were observed,TRAP-positive cells in each ... |
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| Keywords: | Osteoclasts Bone Resorption Diseases Recombinant RANK Protein |
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