山茱萸总苷对去势大鼠骨代谢和骨密度影响的实验研究

[目的]以去势大鼠为研究对象,通过观察骨密度和骨代谢相关的生化指标的变化以探讨"益肾养肝口服液"主药山茱萸的主要有效成分——山茱萸总苷对骨质疏松症的治疗机制。[方法]将50只Wister大鼠随机分为5组,即假手术组、模型组、山茱萸总苷高剂量组、山茱萸总苷中剂量组及山茱萸总苷低剂量组。造模后,山茱萸总苷组依剂量每天灌胃给药。以上各组处理60d,第61天取血,检测血钙(Ca)、血磷(P)、雌二醇(E2)。各组大鼠在灌胃满3个月后取股骨,进行骨密度实验。[结果]山茱萸总苷各剂量组雌二醇(E2)相对于模型组均明显提高,各组的血钙(Ca)、血磷(P)无明显差异。山茱萸总苷各剂量组骨密度明显优于模型组。[结论]山茱萸总苷对去势后大鼠的骨代谢和骨密度有良性调整作用。

Effect of cornel total glycoside on bone metabolism and bone density in caponized rats

Objective To explore the mechanism of cornel total glycoside,the main effective component in Yishen Yanggan for oral use,in treating osteoporosis. Methods Wistar rats were randomly divided into five groups: sham operated group,model group,the high,middle and low dosage of cornel total glycoside group. Ca,P,E2 were determined and bone density experiment was carried out. Results The E2 content and bone density of cornel total glycoside group were obviously higher than that of model group. The blood Ca and P of cornel total glycoside group had not obvious difference when compared with that of model group. Conclusions The cornel total glycoside has bitter regulative effect on bone metabolism and bone density of caponized rats.