| 乙型肝炎表面抗原124aa分子在大肠杆菌中的高效表达 |
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| 引用本文： | 房德兴,李法卿,谭维国,陈华标,金慧英,李素芹. 乙型肝炎表面抗原124aa分子在大肠杆菌中的高效表达[J]. 免疫学杂志, 1999, 15(3): 180-182 |
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| 作者姓名： | 房德兴 李法卿 谭维国 陈华标 金慧英 李素芹 |
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| 作者单位： | 南京军区军事医学研究所,南京,210002 |
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| 摘 要： | 利用聚合酶链反应特异性扩增编码乙型肝炎表面抗原羧基末端１２４个氨基酸的基因片段，并将其大肠杆菌中获得了高效表达。表达产物氨基末端有１个含６组氨酸肽段的２０氨基酸融合部分，分子量１６１７８Ｄａ，以包含体形式存在，约占细菌总蛋白２２％。
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| 关 键 词： | 乙型肝炎病毒 乙型肝炎表面抗原 大肠杆菌 基因表达 |
EXPRESSION OF THE HEPATITIS B SURFACE ANTIGEN FRAGMENT WITH 124 AMINO ACIDS IN E.coli |
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| Fang Dexing,Li Faqing,Tan Weiguo,Chen Huabiao,Jin Huiying,Li Suqin. EXPRESSION OF THE HEPATITIS B SURFACE ANTIGEN FRAGMENT WITH 124 AMINO ACIDS IN E.coli[J]. Immunological Journal, 1999, 15(3): 180-182 |
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| Authors: | Fang Dexing Li Faqing Tan Weiguo Chen Huabiao Jin Huiying Li Suqin |
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| Abstract: | The gene fragment coding the 124 amino acids of the carboxyl terminus of hepatitis B surface antigen(HBsAg)was amplified by polymerase chain reaction(PCR)and the shorted HBsAg was expressed in E.coli in a fusion protein fashion.The fusion protein was 16178Da in molecular weight and contained a 20 amino acid fusion portion at its amino terminus which contained a six histidine peptide segment.The expressed proteins aggregated into inclusion bodies weigh about 22% of the total bacteria proteins.It was easily purified with the HiTrap affinity matrix with a purity up to 95%.ELISA detection showed that the protein could be recognized by polyclonal antibodies against HBsAg and monoclonal antibody against HBsAg a determinant. |
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| Keywords: | Hepatitis B virus Hepatitis B surface antigen E.coli Gene expression |
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