N-甲基-D-天门冬氨酸对大鼠视网膜神经节细胞层神经元的损伤作用

目的 探讨N—甲基—D—天冬氨酸(NMDA)对大鼠视网膜神经节细胞层(RGCL)神经元的毒性作用。方法 通过大鼠玻璃体内注入不同浓度的NMDA，观察注射前后不同时间视网膜神经节细胞层的神经元计数。结果 大鼠RGCL神经元计数随NMDA浓度增加及时间延长而逐渐减少。其中以大神经元对NMDA兴奋毒性最敏感。结论 NMDA可导致RGCL神经元损伤，并显示以大神经元最先受损，即与青光跟性视神经损伤相似，此模型可应用于研究青光跟性视神经损伤机制。

NMDA-induced neuron damage of retinal ganglion cell layer in rat

Objective To investigate the neurotoxic effect of N-methyl-D-aspartate on neurons of retinal ganglion cell layer in rat. Methods The right eyes of rats were given intravitreal injections with different dosage of N-methyl-D-aspartate ( NMDA). Neuron counting of RGCL was performed to evaluate neuron loss at different survival time after intervention. Results There was dose- and time-related loss of neurons in retinal ganglion cell layer( RGCL) after NMDA intravitreal injection. Neurons with large diameter were the most sensitive to NMDA-mediated excitotoxity (P <0. 05). Conclusion NMDA excitoxcity could induce the neuron damage in RGCL. The large neurons are predominately sensitive to the damage. It is similar to the pattern of neuron damage seen in glaucomatous optic neuropathy. It is our suggestion that the model above referred could be used to study glaucomatous optic nerve damage.