毛细管气相色谱法测定消肿止痛酊中樟脑与薄荷脑的含量

目的 建立测定消肿止痛酊中樟脑和薄荷脑含量的方法.方法 采用毛细管气相色谱法.色谱柱为PEG-20M石英毛细管柱(30 m×0.25 mm×0.25 μm);载气为氮气;正辛醇为内标;柱温160℃;氢火焰离子检测器;检测器和气化室的温度均为250℃;N2流速3 ml/min;H2流速30 ml/min;空气流速300 ml/min;尾吹气N2流速30 ml/min.结果 樟脑、薄荷脑和正辛醇能达到良好分离;樟脑的线性范围3.00～18.00 μg/ml(r=0.999 2),薄荷脑的线性范围3.20～19.20 μg/ml(r=0.999 5);樟脑的回收率98.5%～101.5%,薄荷脑的回收率97.6%～101.7%.结论 方法简便、准确、专属性强、重复性好,可用于消肿止痛酊的质量控制.

Determination of Camphor and Menthol in Xiaozhongzhitong Tincture by Capillary Gas Chromatography

Objective To establish a method for GC determination of Camphor and Menthol in Xiaozhongzhitong Tincture.Methods Capillary GC was adopted.The internal standard method was employed with octanol as internal standard.The GC system consisted of a flame ionization detector,PEG-20M capillary column(30 m×0.25 mm×0.25 μm),nitrogen as the carrier gas;oven temperature 160℃,detector temperature 250℃,N2:3 ml/min,H2:30 ml/min,Air:300ml/min,made up(N2):30 ml/min.Results Camphor and Menthol could be separated well under the chromatographic conditions.The linear range of Camphor was 3.00～18.00 μg/ml (r=0.999 2),and the linear range of the Menthol was 3.20～19.20 μg/ml(r=0.999 5);the recovery of Camphor was 98.5%～101.5%,and the recovery of Menthol was 97.6%～101.7%.Conclusion The method is simple,sensitive,accurate and has good repeatability,and it can be used to control the quality of Xiaozhongzhitong Tincture.