| 利用原生质体诱变筛选L-亮氨酸高产菌株的研究 |
| |
| 引用本文： | 齐秀兰,李福德,施安然,陈飞. 利用原生质体诱变筛选L-亮氨酸高产菌株的研究[J]. 沈阳药科大学学报, 1993, 0(1) |
| |
| 作者姓名： | 齐秀兰 李福德 施安然 陈飞 |
| |
| 作者单位： | 沈阳药学院微生物制药教研室，沈阳药学院微生物制药教研室，沈阳药学院，沈阳药学院 微生物制药专业53期学生，微生物制药专业54期学生 |
| |
| 摘 要： | 本实验以L-亮氨酸产生菌黄色短杆菌CF-435为出发菌株,制备原生质体,在原生质体形成率和再生率最佳的条件下,利用紫外线、利福平、氯化锂对原生质体进行复合处理,获得再生突变株,从中挑取单独菌落进行摇瓶发酵,筛选出高产菌株UV_3-29,在含有葡萄糖10%的培养基中发酵72h可积累L-亮氨酸26.73mg/ml,比原始菌种产酸量提高26%.
|
| 关 键 词： | L-亮氨酸 原生质体 诱变 |
SELECTION OF HIGH YIELD L-LEUCINE-PRODUCING STRAIN BY PROTOPLAST MUTATION |
| |
| Qi Xiulan Li Fude Shi Anyan Chen Fei Dept.of Genetics and Breeding. SELECTION OF HIGH YIELD L-LEUCINE-PRODUCING STRAIN BY PROTOPLAST MUTATION[J]. Journal of Shenyang Pharmaceutical University, 1993, 0(1) |
| |
| Authors: | Qi Xiulan Li Fude Shi Anyan Chen Fei Dept.of Genetics and Breeding |
| |
| Affiliation: | Qi Xiulan Li Fude Shi Anyan Chen Fei Dept.of Genetics and Breeding |
| |
| Abstract: | Protoplast from Brevibacterium flavum flavum CF-435 was treated with UV combined with rifampin and LiGl in optimal conditions for protoplast preparation and regeneration. Strain UV3-29 was obtained after shaking culture of regenerated mutants in a medium containing 10% of glucose for 72 hrs. 26.73mg/ml of L-leucine was produced which is an increase of 26% with the original as 100%. |
| |
| Keywords: | L-Leucine Protoplast Mutation |
| 本文献已被 CNKI 等数据库收录！ |
