Hawthorn (<Emphasis Type="Italic">Crataegus monogyna</Emphasis> Jacq.) extract exhibits atropine-sensitive activity in a cultured cardiomyocyte assay |
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Authors: | Satin Salehi Shannon R Long Philip J Proteau Theresa M Filtz |
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Institution: | (1) Department of Pharmaceutical Sciences, Oregon State University College of Pharmacy, 203 Pharmacy Bldg., Corvallis, OR 97331, USA |
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Abstract: | Hawthorn (Crataegus spp.) plant extract is used as a herbal alternative medicine for the prevention and treatment of various cardiovascular diseases.
Recently, it was shown that hawthorn extract preparations caused negative chronotropic effects in a cultured neonatal murine
cardiomyocyte assay, independent of beta-adrenergic receptor blockade. The aim of this study was to further characterize the
effect of hawthorn extract to decrease the contraction rate of cultured cardiomyocytes. To test the hypothesis that hawthorn
is acting via muscarinic receptors, the effect of hawthorn extract on atrial versus ventricular cardiomyocytes in culture
was evaluated. As would be expected for activation of muscarinic receptors, hawthorn extract had a greater effect in atrial
cells. Atrial and/or ventricular cardiomyocytes were then treated with hawthorn extract in the presence of atropine or himbacine.
Changes in the contraction rate of cultured cardiomyocytes revealed that both muscarinic antagonists significantly attenuated
the negative chronotropic activity of hawthorn extract. Using quinuclidinyl benzilate, l-benzylic-4,4′-3H] (3H]-QNB) as a radioligand antagonist, the effect of a partially purified hawthorn extract fraction to inhibit muscarinic receptor
binding was quantified. Hawthorn extract fraction 3 dose-dependently inhibited 3H]-QNB binding to mouse heart membranes. Taken together, these findings suggest that decreased contraction frequency by hawthorn
extracts in neonatal murine cardiomyocytes may be mediated via muscarinic receptor activation. |
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Keywords: | Hawthorn Neonatal cardiomyocyte Atropine Himbacine [3H]-QNB |
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