葛根素通过线粒体途径诱导肺动脉平滑肌细胞凋亡

目的:探讨线粒体途径在葛根素(PUE)诱导肺动脉平滑肌细胞凋亡中的作用.方法:葛根素高、中、低剂量(1.5×104,1.5×10-4,1.5×10-5 mol·L-1)干预体外培养的大鼠肺动脉平滑肌细胞(PASMC),观察线粒体膜电位的变化,Western Blot检测凋亡相关基因Caspase-9,Bax和Bcl-2的蛋白表达.结果:葛根素加药组与正常组相比线粒体膜电位明显降低,葛根素可使Caspase-9的蛋白表达增强,Bcl-2蛋白表达下降而Bax蛋白表达升高,葛根素对PASMC的诱导作用还具有浓度依赖性.结论:葛根素可通过线粒体途径诱导PASMC凋亡.

Puerarin induced apoptosis of pulmonary artery smooth muscle cell by mitochondrial pathway

Objective: To investigate the contribution of mitochondrial pathway in the apoptosis induced by puerarin (PUE) in pulmonary artery smooth muscle cells. Method: Cultured rat pulmonary artery smooth muscle cells (PASMC) were intervened by high, middle and low dose of puerarin (1.5×10^-3, 1.5×10^-4, 1.5×10^-5 mol·L^-1). The change of mitochondrial membrane potential was observed. Western blot detected the expression of apoptosis-related gene Caspase-9, Bax and Bcl-2 protein. Result: Compared with the control group, mitochondrial membrane potential significantly decreased in puerarin groups. Puerarin can enhance the expression of Caspase-9 and Bax protein, decrease the expression of Bcl-2 protein. Puerarin also has a concentration-dependent on the induction of PASMC. Conclusion: Puerarin can induce PASMC apoptosis through mitochondrial pathway.