D2-40标记微淋巴管密度和血管内皮生长因子D在喉癌中的表达及临床意义

目的研究单克隆抗体D2-40标记的微淋巴管密度（lymphatic microvessel density,LMVD）和血管内皮生长因子D（VEGF-D）在喉鳞状细胞癌（简称喉癌）及癌旁组织中的表达情况,探讨两者与喉癌的发生、发展和淋巴结转移的关系及临床意义。方法选择46例喉癌患者手术切除标本,14例癌旁组织标本。采用免疫组化SP法,观察VEGF-D和LMVD在喉癌及癌旁组织中的表达情况,并结合相关临床病理参数进行分析。结果 VEGF-D在喉癌组织中的阳性表达率为73.9%,高于癌旁组织的表达率14.3%,差异有统计学意义（P〈0.05）。VEGF-D的表达与临床分期、病理分级、淋巴结转移有关（P〈0.05）,LMVD则与肿瘤的原发部位、临床分期、淋巴结转移有关（P〈0.05）。生存分析显示VEGF-D阳性表达和阴性表达与患者生存率差异有统计学意义（P〈0.05）。结论 VEGF-D和LMVD在喉癌组织中的表达均增高,VEGF-D促进喉癌组织中LMVD的生成,两者在喉癌的发生发展及淋巴结转移中可能起重要作用。

Expression and their clinical significance of lymphatic microvessel density marked by D2-40 and VEGF-D in laryngeal carcinoma

OBJECTIVE To study the expression of lymphatic microvessel density by the means of D2-40 and vascular endothelial growth factor D(VEGF-D) in laryngeal squamous cell carcinoma and adjacent tissue,and to discuss their clinical significance and relationship with the development of laryngeal carcinoma.METHODS To examine the expression of VEGF-D and lymphatic microvessel density by using immunohistochemistry technique in 46 samples of laryngeal carcinoma,14 samples of adjacent tissue of laryngeal carcinoma and a...