| HPLC法测定乳香药材中11-羰基-β-乙酰乳香酸的含量 |
| |
| 引用本文： | 孔静,于海云,耿桂霞,翟海民.HPLC法测定乳香药材中11-羰基-β-乙酰乳香酸的含量[J].齐鲁药事,2007,26(7):403-404. |
| |
| 作者姓名： | 孔静 于海云 耿桂霞 翟海民 |
| |
| 作者单位： | 山东齐都药业有限公司,山东,淄博,255400 |
| |
| 摘 要： | 目的测定乳香药材中11-羰基-β-乙酰乳香酸的含量,建立有效方法。方法采用HPLC法,二极管阵列检测器;Empower色谱工作站;用十八烷基硅烷键合硅胶为填充剂;C18柱(250×4.6mm,5μm);以乙腈-水-冰乙酸(79∶21∶0.1)为流动相;检测波长为249nm。结果11-羰基-β-乙酰乳香酸在12μg.mL-1~495μg.mL-1(r=0.9999)范围内线性关系良好,平均回收率为100.12%,RSD为1.51%(n=6)。结论所测11-羰基-β-乙酰乳香酸含量符合标准,本法可作为11-羰基-β-乙酰乳香酸的质量控制方法。
|
| 关 键 词： | 乳香 11-羰基-β-乙酰乳香酸 高效液相色谱 |
| 文章编号： | 1672-7738（2007）07-0403-02 |
Determination of acetyl-11-keto-β-boswellic acid in boswellia by HPLC |
| |
| KONG Jing,YU Hai-yun,GENG Gui-xia,ZHAI Hai-min.Determination of acetyl-11-keto-β-boswellic acid in boswellia by HPLC[J].qilu pharmaceutical affairs,2007,26(7):403-404. |
| |
| Authors: | KONG Jing YU Hai-yun GENG Gui-xia ZHAI Hai-min |
| |
| Institution: | Shandong qidu pharmaceutical limited company,Zibo 255400 |
| |
| Abstract: | OBJECTIVE To establish an HPLC method for determination of 11-Keto-β-boswellic acid in boswellia.METHODS The HPLC method was performed on C18 column,acetonitrile-water-Acetic acid;(79∶21∶0.1)as mobile phase,the detection wavelength was 249nm.RESULTS The linear range of acetyl-11-keto-β-boswellic acid was 12μg·mL-1～495μg·mL-1(r=0.9999),The average recovery was 100.12% and RSD was 1.51%(n=6).CONCLUSION The method is simple,highly repeatable,highly sensitive and specific,and can be used for quality control of acetyl-11-keto-β-boswellic acid. |
| |
| Keywords: | boswellia acetyl-11-keto-β-boswellic acid HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
