| 荧光定量聚合酶链反应技术检测孕妇外周血中游离胎儿DNA的可行性研究 |
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| 引用本文： | 贾颐舫,吴爱华,张爱东,杨丹彤,邱毅. 荧光定量聚合酶链反应技术检测孕妇外周血中游离胎儿DNA的可行性研究[J]. 中国计划生育学杂志, 2010, 18(2): 100-102 |
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| 作者姓名： | 贾颐舫 吴爱华 张爱东 杨丹彤 邱毅 |
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| 作者单位： | 山东省优生技术重点实验室,山东省计划生育科学技术研究所,济南,250002 |
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| 基金项目： | 山东省人口和计划生育科研发展项目(项目编号:2003-7) |
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| 摘 要： | 目的:探讨荧光定量聚合酶链反应(FQ-PCR)技术用于孕妇外周血浆中游离胎儿DNA检测的可行性。方法:利用FQ-PCR技术特异性扩增Y基因性别决定区(SRY)并定量分析妊娠中期(18～25周)孕妇血浆中含量。结果:148例男胎孕妇血浆标本中,SRY基因检出率为98.65%。139例女胎孕妇血浆标本中未检测到SRY基因。结论:FQ-PCR技术在孕妇外周血游离胎儿DNA的检测中具有较高的灵敏性和特异性,FQ-PCR技术可应用于非创伤性产前诊断。
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| 关 键 词： | 胎儿DNA 荧光定量聚合酶链反应 非创伤性产前诊断 |
Application of Quantitative Fluorescent Polymerase Chain Reaction in the Measurement of Cell -Free Fetal DNA in the Maternal Circulation |
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| Jia Yifang,Wu Aihua,Zhang Aidong,et al.. Application of Quantitative Fluorescent Polymerase Chain Reaction in the Measurement of Cell -Free Fetal DNA in the Maternal Circulation[J]. Chinese Journal of Family Planning, 2010, 18(2): 100-102 |
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| Authors: | Jia Yifang Wu Aihua Zhang Aidong et al. |
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| Affiliation: | Jia Yifang,Wu Aihua,Zhang Aidong,et al.Sh,ong Provincial Family Planning Science , Technology Institute,Jinan 250002 |
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| Abstract: | Objective: To explore the application value of quantitative fluorescent polymerase chain reaction(FQ-PCR) in the measurement of cell-free fetal DNA in the maternal circulation.Methods: The sex-determining region Y(SRY) gene of cell-free fetal DNA in the maternal(gestational age of 18 to 25 weeks) circulation was detected with FQ-PCR.Results:The SRY gene was detected in the 98.65% of the samples from 148 women who had the male fetuses.None of the SRY gene was detected in the samples from 139 women who had th... |
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| Keywords: | Fetal DNA Quantitative fluorescent polymerase chain reaction Non-invasive prenatal diagnosis |
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