格林-巴利综合征患者的HLA基因分型

目的 观察格林－巴利综合征（ＧＢＳ）患者及其亚组的ＨＬＡ基因分布与正常对照的差异，以寻找ＧＢＳ的遗传易感性及其与ＧＢＳ自身免疫因素的关系。方法 采用参照Ｏｌｅｒｕｐ等的文献合成的序列特异酌情处理寡核苷酸引物，用ＰＣＲ－ＳＳＰ方法对４７例ＧＢＳ患者、７例ＣＪ肠炎和５０例正常对照进行ＨＬＡ分型。结果 在整个ＧＢＳ组、ＣＪ组、有ＣＪ近期感染的ＧＢＳ组、ＩｇＧ型和ＩｇＭ型ＧＭ１抗体阳性的ＧＢＳ组，ＨＬＡ－

HLA Alleles in Patients with Guillain-Barre Syndrome

Objective To stady whether genetic factors may play in concert with autoimmunological factors in the pathogenesis of GBS by determining the difference of HLA alleles between GBS Patients and normal controls. Methods Sequence specific primers of HLA DQA, DQB and DRB alleles were synthesized according to Olerup et al. And HLA was typed by PCR SSP methods in 47 GBS, 7 CJ enteritis patients and 50 normal controls. Results There were no difference of the frequency of HLA DQA, DQB and DRB among GBS group, CJ group, normal controls and GBS subgroups in respect to recent CJ infection,associated with GM1 IgG and GM1 IgM antibodies. But there were increasing tendency of DQA1*0301 in the subgroup with IgG GM1 antibodies ( x 2 =3.659, P =0.051, RR =2.991) and DQA1*0302 in the subgroup with recent CJ infection ( x 2 =2.936, P =0.087, RR =3.587). Conclusions No difference was found among GBS, CJ enteritis patients and normal controls. But the increasing tendency of the two alleles suggests there may be some relation between genetic factors and immunological factors, a definite conclusion waits for further investigations.