HL—60细胞产生的肿瘤免疫抑制因子对T细胞的作用及其生物学特性

白血病细胞培养上清液和白血病人血清能抑制PHA—P诱导的人外周血T淋巴细胞的增殖;抑制白细胞介素2(IL—2)的产生和反应性。以正常2BS人胚肺细胞培养上清液和正常人血清作对照则无抑制作用。白血病细胞培养上清液无细胞毒作用。聚丙烯酰胺凝胶电泳(PAGE)结果表明,来源HL—60人早幼粒白血病细胞的肿瘤免疫抑制因子(tumor-derived immunosuppressive factor,TDSF)的分子量约为87KD,其化学本质为蛋白质。抗急非淋白血病药物对这种TDSF的分泌有部分抑制作用。

Effect of Tumor-derived Immunosuppressive Factor Produced by a HL-60 Cell Line (HL-60) on the Action of T lymphocytes and its Characteristic of Biology

Immunosuppressive activities have been found in the culture supernatant of four leukemiacell lines and in the sera of four patients with leukemia. The inhibitors suppressed PHA-p in-duced lymphocytes proliferation and IL-2 production and responsiveness of T cells to IL-2, butno inhibitory activity was observed in the culture supernatant of 2BS cell line or in the sera ofnormal humam. The culture supernatant of tumor cell lines was not cytoxic. The result of PAGEshows: the molecular weight of TDSF from HL-60 human promyelocytic leukemia is about87KD. It is protein in chemical nature. The synthesis and sercretion of TDSF were partially in-hibited by drugs of anti-acute non-lymphocytic leukemia.