| 大鼠抗—HBs的单克隆抗独特型抗体杂交瘤细胞系的建立 |
| |
| 引用本文： | 高尚先,王玉琴.大鼠抗—HBs的单克隆抗独特型抗体杂交瘤细胞系的建立[J].中国免疫学杂志,1993,9(2):80-83. |
| |
| 作者姓名： | 高尚先 王玉琴 |
| |
| 作者单位： | 中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室，中国药品生物制品检定所细胞室卫生部重点开放实验室 北京，100050，北京，100050，北京，100050，北京，100050，北京，100050，北京，100050，北京，100050，北京，100050 |
| |
| 摘 要： | 用纯化的小鼠单克隆抗—HBs和纯化的大鼠多克隆抗—HBs免疫LOU/C大鼠,取免疫大鼠脾细胞与IR983F骨髓瘤细胞融合,获得两株(TD_1、TD_2)持续分泌抗—HBs的单克隆抗独特型抗体(抗—Id,Ab_2)杂交瘤细胞系。对TD_2进行了较全面的鉴定,TD_2的Ig类型为IgG,亚类为IgG_(2?),核型分析结果染色体数为65条。通过ELISA竞争抑制和中和抑制试验表明,TD_2所分泌的Ab_2既能被不同来源的抗—HBs所中和。又能被HBsAg.所竞争,且都呈现剂量依赖关系。将纯化的TD_2IgG免疫同系大鼠,诱导出了具有抗—HBs活性的抗—抗—独特型抗体(Ab_3)。上述结果证实,TD_2所分泌的单克隆抗体(McAb)是带有HBsAg表位内影像,具有HBsAg免疫源性的抗—HBs的单克隆抗—Id。
|
| 关 键 词： | 单克隆抗体 乙型肝炎 疫苗 |
Production and Identification of Rat-Rat Monoclonal Anti-idiotypic Antibody against Anti-HBs |
| |
| GAO Shang-xian,et al..Production and Identification of Rat-Rat Monoclonal Anti-idiotypic Antibody against Anti-HBs[J].Chinese Journal of Immunology,1993,9(2):80-83. |
| |
| Authors: | GAO Shang-xian |
| |
| Institution: | GAO Shang-xian,et al. Division of Cytology,National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050 |
| |
| Abstract: | Two hybridomas (TD_1, TD_2)secreting monoclonal anti-idiotypic antibodies (anti-ld) againstanti-HBs were established by fusing the myeloma cells, IR983F, with the spleen cells of LOU/Crat which had been immunized with mouse monoclonal anti-HBs to the a determinates and syn-genic rat polyclonal anti-HBs. TD_2(Ab_2)showed the ability to inhibit the binding of horse anti-HBs with HBsAg and was also neutralized by anti-HBs from various origins. Ab_2 can induce an-ti-anti-ld (Ab_3)when injected it into LOU/C rats introperitoneally. Ab_3 can react specifically withHBsAg. These data indicate that TD_2 monoclonal anti-ld against anti-HBs are the anti-ld bearingthe internal image of HBsAg epitope that could mimic HBsAg and possess the immunogenicity ofHBsAg. |
| |
| Keywords: | Anti-idiotype antibody Monoclonal antibody HB vaccine Rat |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
