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| 四种方法检测伯氏疏螺旋体IgG抗体比较 | |
| 引用本文： | 赵文明 刘振龙. 四种方法检测伯氏疏螺旋体IgG抗体比较[J]. 实验动物与比较医学, 1999, 19(1): 29-30 |
| 作者姓名： | 赵文明 刘振龙 |
| 作者单位： | 首都医科大学微生物学教研室 |
| 摘 要： | 用Ｄｏｔ－ＥＬＩＳＡ法、ＥＬＩＳＡ法、ＩＦＡ法及ＣＦ法进行了检测兔莱姆病血清ＩｇＧ抗体的比较实验。对１０例人工感染伯氏疏螺旋体（莱姆病病原体）的兔血清的检测结果表明，Ｄｏｔ－ＥＬＩＳＡ法与ＥＬＩＳＡ法，ＩＦＡ法及ＣＦ法的符合率分别为１００％、９０％和７０％；对１２例正常兔血清的检测，符合率均为１００％。这提示，Ｄｏｔ－ＥＬＩＳＡ法可能成为一种用于检测人或动物莱姆病血清抗体的简便方法。 |
| 关 键 词： | 莱姆病 斑点－酶联免疫吸附试验 酶联免疫吸附试验 间接免疫荧光试验 补体结合试验 |
Comparative Study on Four Methods for Detecting Serum Antibody against Borrelia Burgdorferi |
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| Zhao Wenming Liu Zhenlong Shen Haizhong. Comparative Study on Four Methods for Detecting Serum Antibody against Borrelia Burgdorferi[J]. Laboratory Animal and Comparative Medicine, 1999, 19(1): 29-30 | |
| Authors: | Zhao Wenming Liu Zhenlong Shen Haizhong |
| Abstract: | Serum IgG antibody from rabbits artificially infected with Borrelia burgdorferi was detected with new-established Dot-ELISA,routine ELISA,IFA and CF,respectively.Ten serum samples from infected rabbits were examined,the results of Dot-ELISA showed 100%,90% and 70% coincidence with routine ELISA,IFA and CF respectively,while to 12 serum samples from healthy rabbits,it showed 100% coincidnce with ELISA,IFA and CF.All these suggested that Dot-ELISA may be a simple and convenient method for detecting serum IgG antibody against human and animal Lyme disease. |
| Keywords: | Lyme disease Dot-ELISA ELISA IFA CF |
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