中药清热凉血新法治疗温病湿热证的微观实验观察

目的:探讨清热解毒、凉血化瘀对常见病证温病湿热证的作用,并从微观角度探讨温病湿热证血瘀的客观性。方法:采用复合因素复制的新西兰兔温病湿热证模型,设立黄连解毒汤(HuanglianJiedudecoction,HJD)组、黄连解毒汤加丹参(Salviamiltior-rhiza,SM)、赤芍(Paeonialactiflora,PL)(HJD SM PL)组、王氏连朴饮(WangshiLianpudrink,WLD)组、王氏连朴饮加丹参、赤芍(WLD SM PL)组、湿热证模型组、正常对照组,观察各组症状及体温变化、血浆内毒素、肿瘤坏死因子(TNF)、白细胞介素-1(IL-1)、血液流变学、血小板、凝血功能等指标变化。结果:HJD SM PL组症状表现较轻,HJD组及HJD SM PL组体温升高较小,药物治疗各组内毒素犤HJD,HJD SM PL,WLD,WLD SM PL组分别为(0.57±0.06),(0.56±0.07),(0.63±0.03),(0.62±0.02)μg/L〗,TNF,IL-1较湿热模型组犤内毒素为(0.79±0.05)μg/L〗显著降低(P<0.05～0.01),HJD组、HJD SM PL组内毒素,IL-1的含量尚低于WLD组,差异有显著性意义(P<0.05)。湿热模型组血液流变学各参数与正常对照组比较,有非常显著性意义(P<0.01)。HJD SM PL组、WLD SM PL组在改善上述指标效果较好,优于HJD组、WLD组,差异有显著性意义(P<0.05～0.01)。结论:清热解毒对于温病湿热证具有较好的治疗作用,可以缩短

Experimental study of treatment of damp-heat syndrome of seasonal febrile disease with traditional Chinese medicine to clear away heat and cool blood

AIM: To explore the effects of clearing away heat and removing toxic substances(CHRTS) and cooling blood to dissipate blood stasis(CBDBS) on damp-heat syndrome of seasonal febrile disease (DHSSFD), which is common in clinic, and to study objectivity of blood stasis of DHSSFD from microcosmic angle.METHODS: New Zealand rabbit DHSSFD models were established by compound factors and randomly divided into Huanglian Jiedu decoction group(HJD, group A), Huanglian Jiedu decoction plus salvia miltiorrhiza (SM) and peeonia lactiflora(PL) group(group B), Wangshi Lianpu drink group(WLD, group C), Wangshi Lianpu drink plus SM and PL group(group D), DHSSFD gronp(gronp E), normal control group(group F). The changes in following parameters, including symptom and temperature, plasma levels of endotoxin(ET), tumor necrosis factor(TNF), interlenkin-1 (IL-1), platelet,thrombin parameters and bemorheological parameters.RESULTS: Compared with model group, group B has a slighter symptom. There was a smaller increase in temperature in group A and group B. The contents of ET, TNF and IL-1 in plasma in every treatment group decreased significantly than that in the model group( P ＜ 0. 05 - 0. 01 ). The contents of ET, IL-1 in plasma in group A and group B significantly lower than that in group C. Hemorrheology parameters of model group have significant difference compared with normal control group. Group B and group D have better curative effect than group A and group C on the mentioned parameters( P ＜ 0. 05 - 0.01 ).CONCLUSION: The therapy of CHRTS has a better curative effect on DHSSFD and can shorten the course of disease. Microcosmic blood stasis of DHSSFD exists objectively without traditional four kinds diagnosis, and better efficacy might be obtained when treated with the drugs of BDBS.