小型猪腮腺放射损伤唾液流率和血常规及生化动态观察

目的 :动态观察小型猪腮腺放射损伤前后腮腺流率、血常规及血生化变化 ,为建立小型猪腮腺放射损伤动物模型 ,及基因治疗涎腺放射损伤奠定基础。方法 :试验组 8只小型猪 ,1 5Gy单侧腮腺放射损伤 ,对侧腮腺做对照 ,2只 0Gy放射做空白对照。放疗前、放疗后 4周、8周和 1 6周 ,用吸盘法收集唾液 ,前腔静脉取血 ,观察唾液流率、血常规和血生化改变。结果 :放疗后 8周唾液流率下降 ,1 6周唾液流率下降明显并与放疗前有显著性差别。随着放射后的时间延长 ,白细胞计数下降越来越明显 ,第 8周和 1 6周与放疗前有显著性差别。红细胞在放疗后第 8周及第 1 6周稍有增加但没有显著性差别 ,血小板在 4周下降 ,第 8周及第 1 6周增高超出放射前水平。乳酸脱氢酶血清含量放疗后呈持续下降趋势 ,与放疗前有显著性差别(P <0 0 5 )。血清淀粉酶在放疗后 1周增高 ,第 4周显著下降 ,与放疗前相比差别有显著性 ,随后缓慢回升。结论 :1 5Gy放疗1 6周时放疗侧腮腺唾液流率明显下降 ,血液常规白细胞计数和血清乳酸脱氢酶、淀粉酶改变

EFFECT OF IRRALIATION TO PAROTID GLAND OF MINIATURE PIG ON THE PAROTID FLOW RATE,BLOOD ROUTINE TEST AND SERUM CHEMISTRY

Objective:To study the trending changes of minipig parotid salivary flow rate,blood routine examination and serum chemistry after irradiation (IR) damage to parotid gland.Methods:Ten minipigs were divided into 2 groups.Unilaterial,single dose irradiation (15Gy) was delivered via a liner accelerator to one side of parotid glands of eight minipigs,blank control to two minipigs.Saliva collections were done in pro IR,IR+4weeks,IR+8weeks,IR+16weeks.Blood was drawn at the same timepoints.Results:At 8 weeks post IR,decreased parotid saliva flow rates was found in the irradiated side,there was significant difference between the flow rates of 16weeks post IR and pro IR (P<0 05).At post IR,WBC count decreased.There was significant difference of WBC count between at 8weeks,16weeks post IR and pro IR.Compared with pro IR,LDH of serum significantly decreased after irradiation.Conclusion:At 16weeks post IR,saliva flow rate significantly decreased in irradiated parotid gland (15Gy).Compared with pro IR,at post IR WBC count and LDH and AMY of serum significantly changed.