| 两种精子优选法对精子运动参数、活率影响 |
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| 引用本文： | 王洪亮,刘睿智,沙艳伟,薛百功. 两种精子优选法对精子运动参数、活率影响[J]. 中国实验诊断学, 2005, 9(1): 137-138 |
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| 作者姓名： | 王洪亮 刘睿智 沙艳伟 薛百功 |
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| 作者单位： | 1. 吉林大学第一临床医院,泌尿外科,吉林,长春,130021
2. 吉林大学基础医学院,细胞生物学教研室 |
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| 基金项目： | 吉林省科技厅资助项目(20030433) |
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| 摘 要： | 目的 评价Percoll密度梯度法和上游法两种精子分离方法对精子运动参数和活率影响。方法 应用计算机精液分析仪进行精子运动参数分析 ,伊红 Y染色分析精子活率。结果 Percoll和上游两种方法优选后精子运动能力和活率均明显高于优选前 (P <0 0 0 1)。结论 Percoll和上游法优选后精子运动能力和活率增加。
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| 关 键 词： | 精子运动参数 精子优选法 活率 |
| 文章编号： | 1007-4287(2005)01-0137-02 |
| 修稿时间： | 2004-08-18 |
The effect of Sperm Selection Technique Methods on Motility parameters of Human Spermatozoa |
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| WANG Hong liang ,LIU Rui zhi ,SHA Yan wei ,et al.. The effect of Sperm Selection Technique Methods on Motility parameters of Human Spermatozoa[J]. Chinese Journal of Laboratory Diagnosis, 2005, 9(1): 137-138 |
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| Authors: | WANG Hong liang LIU Rui zhi SHA Yan wei et al. |
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| Affiliation: | WANG Hong liang 1,LIU Rui zhi 2,SHA Yan wei 2,et al. |
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| Abstract: | Objective To study the effect of semen selection technique method on motility parameters and viability of human spermatozoa. Methods The sperm motility parameters were analyzed by computer assisted semen analyzer(CASA). Sperm viability was analyzed by eosin Y staining. Results Post semen selection sperm motility and viability were significantly higher than those before( P <0 001, P <0 05), whereas there were no statistics differences( P >0 05) in sperm motility between Percoll selection and swimming selection. Conclusion semen selection technique increase sperm motility and viability. |
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| Keywords: | sperm semen selection Technique sperm motility Viability |
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