| RP-HPLC监测重组人粒细胞集落刺激因子复性动态过程 |
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| 引用本文： | 钟英!杭州.RP-HPLC监测重组人粒细胞集落刺激因子复性动态过程[J].中国药学杂志,1999,34(12):834-836. |
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| 作者姓名： | 钟英!杭州 |
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| 作者单位： | 杭州九源基因工程有限公司 |
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| 摘 要： | 目的:建立监测重组人粒细胞集落刺激因子(rhG-CSF)复性动态过程的方法?方法:反相高效液相色谱法?采用VydacC4(150mm×4.6mm)柱,流动相为三氟醋酸-乙腈混合物梯度洗脱?结果:发现完全复性有生物学活性的rhG-CSF在25.4min左右出峰,而完全变性无生物学活性的rhG-CSF在26.4min左右出峰,两峰出峰时间相差1min左右?随着复性时间的增加,两者的峰面积之比在变化?Tris复性体系与PB复性体系相比,复性收率差不多,都可在12h内达到的70%以上复性正确率?结论:此方法适用于基因工程产品纯化过程中复性产率的控制?
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| 关 键 词： | 重组人粒细胞集落刺激因子 反相高效液相色谱法 复性 |
| 收稿时间： | 1999-03-05; |
Reversed phase liquid chromatography as a tool for rhG CSF refolding |
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| Zhong Ying.Reversed phase liquid chromatography as a tool for rhG CSF refolding[J].Chinese Pharmaceutical Journal,1999,34(12):834-836. |
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| Authors: | Zhong Ying |
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| Abstract: | OBJECTIVE:To develop an assay in order to monitor the kinetic processes of refolding of recombinant human granulocyte colony stimulating factor.METHOD:A reversed-phase high-perfermance liquid chromatography was developed.Vydac C4(150 mm×4.6 mm) column was used and the mobile phase consisted of a mixture of trifluoroacetic acid-acetonitrile.RESULTS:The correct folding peaks and denatured peaks were separated well.The peak appeared on 25.4 min had biology activity.CONCLUSION:This method may be used for the measurements of correct folding rate of rhG-CSF. |
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| Keywords: | recombinant human granulocyte colony stimulating factor RP HPLC folding |
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