| Effects of Electromagnetic Field and Basic Fibroblast Growth Factor on Osteoblast＇s Growth |
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| 引用本文： | GUOYong ZHANGXi-zheng WANGHao LIBin LIRui-xin WUJin-hui ZHAOYun-shan WUJi-min. Effects of Electromagnetic Field and Basic Fibroblast Growth Factor on Osteoblast＇s Growth[J]. 中国生物医学工程学报(英文版), 2004, 13(3): 133-138 |
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| 作者姓名： | GUOYong ZHANGXi-zheng WANGHao LIBin LIRui-xin WUJin-hui ZHAOYun-shan WUJi-min |
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| 作者单位： | InstituteofMilitaryMedicalEquipment,AcademyofMilitaryMedicalSciences.TianJin300161,China |
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| 基金项目： | This work Supported by National Natural Science Foundation( 10 172 0 93) and the10 th Five-Year PlanMedical Research Foun-dation of PL A( 0 1MA0 88) |
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| 摘 要： | Osteoblasts of rat cultured in vitro were stimulated with pulsed 50 Hz electromagnetic field and basic fibroblast growth factor(bFGF). The MTT method, flow cytometry and histochemistry staining were used to detect cell proliferation, cell cycle and alkaline phosphatase. The results indicated : after stimulated by 1 mT electromagnetic field, the cells are more abundant,have more S phase percentages, 2 mT electromagnetic field have no evident effect on cells‘ growth;compared with electromagnetic field, the cells stimulated by bFGF are more abundant and have larger S phase ratios. Electromagnetic field and bFGF have no effect on cells, alkaline phosphatase. Therefore ,we concluded that electromagnetic field can enhance osteoblasts growth like some growth factor such as basic fibroblast growth factor, and the osteoblasts‘, characteristics was not changed.
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| 关 键 词： | 造骨细胞 细胞生长 纤维原细胞生长因子 电磁场 |
Effects of Electromagnetic Field and Basic Fibroblast Growth Factor on Osteoblast's Growth |
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| GUO Yong,ZHANG Xi-zheng,WANG Hao,LI Bin,LI Rui-xin,WU Jin-hui,ZHAO Yun-shan,WU Ji-min Institute of Military Medical Equipment,Academy of Military Medical Sciences,TianJin ,China. Effects of Electromagnetic Field and Basic Fibroblast Growth Factor on Osteoblast's Growth[J]. Chinese Journal of Biomedical Engineering, 2004, 13(3): 133-138 |
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| Authors: | GUO Yong ZHANG Xi-zheng WANG Hao LI Bin LI Rui-xin WU Jin-hui ZHAO Yun-shan WU Ji-min Institute of Military Medical Equipment Academy of Military Medical Sciences TianJin China |
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| Affiliation: | GUO Yong,ZHANG Xi-zheng,WANG Hao,LI Bin,LI Rui-xin,WU Jin-hui,ZHAO Yun-shan,WU Ji-min Institute of Military Medical Equipment,Academy of Military Medical Sciences,TianJin 300161,China |
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| Abstract: | Osteoblasts of rat cultured in vitro were stimulated with pulsed 50 Hz electromagnetic field and basic fibroblast growth factor(bFGF).The MTT method,flow cytometry and histochemistry staining were used to detect cell proliferation?cell cycle and alkaline phosphatase .The results indicated: after stimulated by 1 mT electromagnetic field,the cells are more abundant,have more S phase percentages,2 mT electromagnetic field have no evident effect on cells'growth;compared with electromagnetic field,the cells stimulated by bFGF are more abundant and have larger S phase ratios.Electromagnetic field and bFGF have no effect on cells,alkaline phosphatase.Therefore ,we concluded that electromagnetic field can enhance osteoblasts growth like some growth factor such as basic fibroblast growth factor,and the osteoblasts',characteristics was not changed. |
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| Keywords: | Electromagnetic field Basic fibroblast Growth factor Osteoblast |
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