血小板激活因子薄层层析分离和生物法测定的应用体会

目的：介绍作者应用薄层层析分离和生物活测定法测定血小板激活因子的体会，重点讨论分离和测定过程中可能出现的问题及注意事项。方法和结果：采用上述方法测定猪急性重症胰腺炎时门静脉血标本，结果显示，血小板激活因子的浓度较对照组明显升高，结论：薄层层析分离和生物测定的方法适用于血小板激活因子的检测，只要在检测过程中注意操作的合理性与规范性,检测结果就可满意。

Experience of thin layer chromatography separation and bioassay of platelet activating factor

Objectives:To introduce the author's experiences in bioassay of platelet activating factor with thin layer chromatographic separation and platelet aggregation method, and to discuss probable problems in the process. Methods and Results:The method was used in determining portal venous blood samples from swine models of acute severe pancreatitis, control value were 0.79~1.65 pmol/L, in acute severe pancreatitis, mean blood level of platelet activating factor may be as high as 14.75 pmol/L. Conclusions: Thin layer chromatography separation and platelet aggregation assay were suitable for the determination of platelet activating factor, the results were satisfactory in case of standardized operation.