羟基喜树碱联合吡柔比星治疗涎腺腺样囊性癌肺转移的实验研究

目的研究羟基喜树碱（HCPT）联合吡柔比星（THP）治疗涎腺腺样囊性癌（ACC）的合理用药顺序.方法60只裸鼠建立ACC肺转移动物模型后,将动物随机分为6组,分别按6种用药顺序组合治疗.6周后处死各组动物并取完整肺部标本,经图像分析测量肿瘤面积.结果化疗各组肿瘤面积明显小于空白对照组（P＜0.01）;HCPT和THP同时给药组和两单药组差异无统计学意义（P＞0.05）,但两药顺序给药或间隔48 h给药组与2个单一给药组及两药同时给药组比较,差异有统计学意义（P＜0.05）.结论HCPT联合THP在治疗ACC肺转移时具有协同效应,但应避免同时给药.

Experimental study of hydroxycamptothecine and theprubicine in the treatment of adenoid cystic carcinoma lung metastases

OBJECTIVE: To investigate anticancer effect of hydroxycamptothecine and theprubicine in the treatment of established human adenoid cystic carcinoma (ACC) lung metastasis of nude mice and to determine the best therapy sequence of the two drugs. METHODS: The model of ACC lung metastasis (60 animals) was established by injecting the ACC-Mcells into the nude mice from their tail veins. Animals were divided into six groups: vacuity control group, HCPT group, THP group, HCPT and THP group 1, HCPT and THP group 2, HCPT and THP group 3 (No.1 - No.6). Different treatment sequence was performed on these groups. The lung samples were collected and observed after HE staining. The area of metastases and lung tissue were analyzed by the images analysis system. RESULTS: The size of the metastatic lesion in the treatment groups were smaller than that in the control group (P < 0.01). The treatment effects between the group with simultaneous administration of HCPT and THP and the group with single drug administration were not different (P > 0.05). No.5 and No.6 groups showed better effect of treatment than the other treatment groups (P < 0.05). CONCLUSIONS: There are two recommended sequences: (1) HCPT should be used after THP; (2) THP is used 48 hours, After HCPT is administered. Because using two drugs together will decrease the anticancer effect, HCPT and THP should not be used simultaneously.