| 附子质量生物检定方法探索性研究 |
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| 引用本文： | 代小娇,宋军,鄢良春,赵军宁,易进海. 附子质量生物检定方法探索性研究[J]. 四川中医, 2013, 0(9): 43-46 |
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| 作者姓名： | 代小娇 宋军 鄢良春 赵军宁 易进海 |
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| 作者单位： | [1]四川省中医药科学院,国家中医药管理局中药质量生物评价重点研究室,四川省道地药材系统开发工程技术研究中心,中药品质评价与创新中药研究四川省重点实验室,成都610041 [2]泸州医学院,四川泸州646000 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）,课题编号：2009CB522801; 国家自然基金面上项目,课题编号：81073047 |
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| 摘 要： | 目的：采用离体心脏模型建立附子生物检定方法,优化实验条件。方法：通过比较不同实验条件下附子对心脏的作用,筛选检测指标,优化实验条件,从而建立附子生物检定的检测体系。结果：检测体系条件为：采用Langendorff离体心脏灌注装置,K-H液灌注,采用恒温、恒压、恒量的方法给药,以心率为判定指标。结论：所优化建立的实验体系符合附子生物检定的要求。本法有可能成为现行附子质量控制方法的一种补充。
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| 关 键 词： | 附子 质量评价 生物效检定 |
The Exploratory Study of Bioassay Method on Aconite Quality |
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| Affiliation: | DAI Xiaojiao;SONG Jun;ZHAO Junning;Sichuan Academy of Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine Quality Biological Evaluation of Key Research,the System Development Engineering Research Center of Sichuan Province Authentic Herbs,Sichuan Provincial Key Laboratory of Evaluation of Traditional Chinese Medicine Quality and Innovation of Traditional Chinese Medicine Research;Luzhou Medical College; |
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| Abstract: | Objective： Using the isolated heart model to establish Aconite bioassay method,Optimizing the experimental conditions. Methods： Comparing the aconite＇ s role of the heart with the different experimental conditions,Screening test indicators,Optimizing the experimental conditions,So as to establish the aconite bioassay detection system. Results： Inspection system conditions： Using Langendorff isolated heart perfusion device,K-H liquid perfusion,the use of constant temperature and pressure,a constant amount of administration,heart rate decision index. Conclusion： The optimization of the experimental system establishes to comply with the requirements of aconite bioassay. Law may become a complement of the existing the aconite quality control methods. |
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| Keywords: | Aconite quality evaluation Biological potency test |
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