3种基因工程药物对大鼠动脉阻塞性脑损伤治疗作用的比较研究

目的 :探讨基因工程药物NIF、NHH、TNHH对大鼠动脉阻塞性脑损伤的治疗作用。方法 :用线栓法将24只大鼠制成动脉阻塞性脑缺血损伤模型后分为治疗组 (3组 )和模型对照组 ,每组6只。治疗组静脉给予NIF、NHH、TNHH ,模型组给予生理盐水。分别于术后4、8、24、48、72h进行神经行为学评分 ;术后72h取血测定血清APTT ,判断凝血功能 ;取脑组织行TTC染色 ,计算梗塞灶体积 ;HE染色观察脑组织病理变化。结果 :治疗组各时间点行为学评分均优于模型组 (P<0 05) ,血清APTT比模型组均有明显延长 (P<0 05) ,脑组织梗塞灶体积比模型组小 (P<0 05) ,HE染色脑组织病理变化均较模型组轻。结论 :NIF、NHH、TNHH对大鼠动脉阻塞性脑缺血损伤有明显的保护作用 ,其中以TNHH最优。

Comparison of Protective Effects of Gene Engineering Agents,NIF,NHH or TNHH on Focal Cerebral Ischemia Injury in Rats Subjected to Middle Cerebral Artery Occlusion

OBJECTIVE:To investigate the protective effects of NIF,NHH,TNHH on focal cerebral ischemic injury induced by middle cerebral artery occlusion in rats.METHODS:24 SD rats were randomly divided into 4 groups:control group(n=6)received normal saline 0.1ml/kg,group NIF(n=6),group NHH(n=6)and group TNHH(n=6)received NIF,NHH,TNHH 2mg/kg i.v.(NIF,NHH,TNHH were dissolved in normal saline 2mg/kg)respectively just before middle cerebral artery occlusion(MCAO)with 3～0 nylon monofilament suture for 3 days.The neurological behavior was evaluated at 4hr,8hr,24h,48h,72h after operation.The infarct volume was then assessed after TTC staining following the last neurological behavior evaluation.Two specimens were randomly taken in 4 groups and were sent to HE staining.RESULTS:The neurologic defect score(NDS)of group NIF,NHH,TNHH after operation decreased significantly(P<0.01)compared with that of control group.The infarct volume of control group (39.4±5.7mm3)was significantly greater(P<0.01)than those of group NIF(24.9±9.6mm3),group NHH(25.2±5.2mm3)and group TNHH (23.2±5.4mm3)at 72h after operation.The pathological change of control group was slighter than those of group NIF,NHH,TNHH.CONCLUSION:NIF,NHH,TNHH significantly reduce neurologic injury induced by MCAO.