口服抑肽酶对大鼠急性肝损伤的保护作用

目的：探讨口服抑肽酶对实验大鼠肝损伤的保护作用。方法：Wistar大鼠60只，采用四氯化碳（CCl₄）法建立急性肝损伤实验动物模型，随机分为对照组、模型组及抑肽酶大、中、小剂量组和阳性对照组(甘利欣组)，观察抑肽酶对实验 鼠血清丙氨酸氨基转移酶 (ALT)、天门冬氨酸氨基转移酶(AST)、一氧化氮(NO)、还原型谷胱甘肽(GSH)含量、肝重量系数以及肝组织病理改变的影响。结果：模型组与正常对照组比较，血清ALT及AST均显著增高(P<0.001)、NO明显增高(P<0.01)、肝重量系数明显增加(P<0.001)、GSH明显降低（P<0.01）。抑肽酶小、中、大剂量组与模型组比较：ALT及AST值均显著降低(P<0.001)，GSH值均明显增高 (P<0.01)，与甘利欣组相近；NO值均明显降低(P<0.05)，且低于甘利欣组；肝重量系数均明显降低(P<0.01，P<0.05，P<0.001)。鼠肝组织的病理性损伤改变程度，抑肽酶大、中、小剂量组及模型组依次加重。结论：口服抑肽酶对大鼠急性肝损伤具有明显的保护作用。

Protective effects of oral aprotinin on acute liver injury in rats

Objective To investigate the protective effects of oral aprotinin on acute hepatic injury in rats.(Methods Sixty) Wistar rats were divided into 6 groups:normal control group(Ⅰ),model control group(Ⅱ),low,middle,high doses of aprotinin groups(Ⅲ,Ⅵ,Ⅴ) and diammonium glycyrrhizinate control group(Ⅵ).The changes of activities of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),nitric oxide(NO),glutathione(GSH) and the liver weight coefficent were measured.The pathologic changes of liver tissue were measured.Results The serum ALT,AST,NO concentrations in rats and the liver weight coefficient in Group Ⅱ were increased significantly compared with Group Ⅰ(P<0.001,P<0.001,P<0.01,P<0.001),the serum GSH concentration in rats in Group Ⅱ was decreased significantly compared with Group Ⅰ(P<0.01).The serum ALT,AST,NO concentrations in rats in Group Ⅲ,Group Ⅳ and Group Ⅴ were decreased significantly compared with Group Ⅱ(P<0.001,P<0.001,P<0.05).The serum GSH concentrations in rats in Group Ⅲ,Group Ⅳ and Group Ⅴ were increased significantly compared with Group Ⅱ(P<0.01).The liver weight coefficients in Group Ⅲ,Group Ⅳ and Group Ⅴ were decreased significantly compared with Group Ⅱ(P<0.01,P<0.05,P<0.001).The order of pathologic change levels of liver tissue of acute hepatic injury was Group Ⅴ,Group Ⅳ,Group Ⅲ,Group Ⅱ.Conclusion The oral aprotinin has significantly protective function on rats with acute liver injury.