前列安对良性前列腺增生症大鼠模型前列腺组织碱性成纤维生长因子的影响

目的: 探讨前列安治疗前列腺增生症的作用机制。 方法: 采用大鼠去势后注射丙酸睾酮致前列腺增生法造模。分5组,即正常组、模型组、前列安低剂量0.1 g ·kg^-1组、前列安高剂量0.2 g ·kg^-1组、癃闭舒0.2 g ·kg^-1组,ig给药30 d后处死。摘取大鼠前列腺并用电光分析天平称重,获取前列腺指数,同时制作光镜和免疫组化标本,对每张免疫组化片进行显微定性分析并观察光镜下前列腺细胞形态变化。 结果: 3个治疗组大鼠前列腺重、前列腺指数及碱性成纤维生长因子(bFGF)表达水平均明显低于模型组,其中尤以前列安高剂量组最为明显。模型组大鼠前列腺组织见明显的病理性改变,前列安低剂量组大鼠前列腺组织的病理性改变较模型组减轻,高剂量组和癃闭舒组大鼠前列腺组织的病理改善最为明显。 结论: 前列安可降低大鼠前列腺湿重,修复增生的病理损伤,其作用机制可能与前列安抑制前列腺组织中bFGF释放有关。

Effect of Qianliean on Basic Fibroblast Growth Factorlevelsin in Rats Model of Benign Prostatic Hyperplasia

Objective: To investigate the effect of Qianliean on the treatment of benign prostatic hyperplasia and the related mechanism. Method: Rat model of benign prostatic hyperplasia was established by castration followed by injection of testosterone.The rats were randomly divided into 5 groups:the normal group,the model group,the Qianliean high-dose group,the Qianliean low-dose group,Longbishu group.The rats were treated accordingly and sacrificed after 30 d.The prostates were sampled and weighed,and the prostate index was calculated.Light microscopic and immunohistochemical specimens were prepared and investigated. Result: Prostatic weight,prostatic index and basic fibroblast growth factorlevelsin (bFGF) expression levels in three treatment groups were significantly lower than that of the model group,in particular for the Qianliean of high-dose group.Model group showed marked pathological changes in prostate tissue,Qianliean low-dose group showed alleviated pathological changes in prostate tissue compared with the model group,high-dose group and Longbishu group rats prostate tissue showed obvious improvement in pathological changes. Conclusion: Qianliean can decrease prostate wet weight in rat and alleviate pathological damage in prostate.The mechanism may be related to the inhibition of prostate tissue bFGF release.